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Products:Immunology >> Cell Type Markers >> Non-CD >> APC
Overview
Product name
Anti-Granulocytes antibody [0.N.315]
See all Granulocytes products (9) ...
Description
Mouse monoclonal [0.N.315] to Granulocytes
Specificity
Recognizes an unidentified antigen in the cytoplasm of mature granulocytes.
Tested applications
IHC-P, IHC-Fr, IFmore details
Cross reactivity
Reacts with
Human
Does not react with
Rat
Immunogen
Nuclei from pokeweed mitogen-stimulated human peripheral blood lymphocytes.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.2% BSA, 10mM PBS, pH 7.5
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
0.N.315
Isotype
IgG1
Research areas
Immunology >> Innate Immunity >> Myeloid Cells
Immunology >> Cell Type Markers >> Non-CD >> APC
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-Fr
IF
Application notes
IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 - 4 µg/ml. No special pretreatment is required.
IHC-Fr: Use at a concentration of 2 - 4 µg/ml.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.
References for Anti-Granulocytes antibody [0.N.315] (ab14497)
ab14497 has not yet been referenced specifically in any publications.
Publishing research using ab14497? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"