Anti-Granulocytes antibody [SPM250] (ab75701)

Overview

  • Product nameAnti-Granulocytes antibody [SPM250]
    See all Granulocytes primary antibodies
  • Description
    Mouse monoclonal [SPM250] to Granulocytes
  • Specificityab75701 reacts with early precursor and mature forms of human myeloid cells. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human): Nuclei from pokeweed mitogen stimulated peripheral blood lymphocytes.

  • Positive control
    • Human tonsil or lymph node tissue
  • General notes


    ab75701 can be useful for the detection of myeloid leukemias and granulocytic sarcomas and also can be used as a marker of granulocytes in normal tissues or inflammatory processes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notes ab75701 can be useful for the detection of myeloid leukemias and granulocytic sarcomas and also can be used as a marker of granulocytes in normal tissues or inflammatory processes.
  • Clonality Monoclonal
  • Clone numberSPM250
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab75701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
IHC-P 1/50.

Target

  • RelevanceGranulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.
  • Alternative names
    • PML antibody
    • PMN antibody
    • Polymorphonuclear leukocytes antibody

Anti-Granulocytes antibody [SPM250] images

  • ab75701, at a 1/50 dilution, staining Granulocytes in human tonsil tissue by Immunohistochemistry.

References for Anti-Granulocytes antibody [SPM250] (ab75701)

ab75701 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"