Granzyme A Human ELISA Kit (with color giving dyes) (ab119526)
- Product nameGranzyme A Human ELISA Kit (with color giving dyes)
Intra-assay Sample n Mean SD CV% Overall 8.7% Inter-assay Sample n Mean SD CV% Overall 8.6%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity0.4 pg/ml
- Range0.8 pg/ml - 600 pg/ml
- Species reactivityReacts with: Human
- Product overview
ab119526 Granzyme A Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of Human Granzyme A. ab119526 is for research use only and is not for diagnostic or therapeutic procedures.
Granzymes are exogenous serine proteinases that are released from cytoplasmic granules of cytotoxic lymphocytes (CTLs) and NK cells. The name 'granzymes' is derived from: granules + enzymes. These granules contain next to granzymes other proteins including a poreforming protein (Perforin). Upon binding of the CTL to a target cell (by CTL-receptor and antigen-presenting MHC molecules on the target cell) the contents of the granules are released in the intercellular space where after perforine will perforate the target cell membrane by forming transmembrane pores. Through these pores the granzymes can now enter the cytosol of the target cell. Granzyme B activates the intracellular cascade of caspases finally resulting in the killing of the target cells.
Percentages of the Granzyme A and B positive CTLs can be determined by flow cytometry and immunocytochemical methods for many disorders.
Not all granzymes enter the target cell, part of them also 'leak' in to the peripheral blood and other biological fluids. Detectable amounts of granzymes have been found to circulate in healthy volunteers. These soluble granzymes can be measured by ELISAs. Increased levels of soluble granzymes have been found with patients suspected of an increased NK cell and CTL-response caused by systemic viral infections such as EBV, HIV, CMV, hepatitis A and Dengue fever. Soluble Granzyme A and B is increased in synovial fluid from rheumatoid arthritis and significantly higher than levels in patients with osteoarthrosis. Granzymes are likely involved in the acute rejection of kidney-transplants, as infiltrating lymphocytes in the rejected kidney strongly express granzymes. Increasing plasma levels of soluble granzymes in patients with a kidney transplants.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
- Storage instructionsPlease refer to protocols.
Components 1 x 96 tests Adhesive Films 4 units Biotin-Conjugate anti-human Granzyme A monoclonal antibody 2 x 70µl Blue-Dye 1 x 0.4ml Dilution Buffer Concentrate (5x) 1 x 7ml Green-Dye 1 x 0.4ml Human Granzyme A Standard 2 vials Monoclonal anti-human Granzyme A-coated microwell plate 1 unit Red-Dye 1 x 0.4ml Stop Solution 1 x 15ml Streptavidin-HRP 1 x 150µl Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml
- Research Areas
Contains 1 peptidase S1 domain.
- CTL tryptaseCTLA3Cytotoxic T lymphocyte associated serine esterase 3
- Cytotoxic T-lymphocyte proteinase 1Fragmentin-1GRAA_HUMANGranzyme 1Granzyme AGranzyme-1GZMAH factorHanukkah factorHFHFSP
Our Abpromise guarantee covers the use of ab119526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Granzyme A Human ELISA Kit (with color giving dyes) images
Representative standard curve using ab119526.
References for Granzyme A Human ELISA Kit (with color giving dyes) (ab119526)
ab119526 has not yet been referenced specifically in any publications.