Granzyme A Human ELISA Kit (with color giving dyes) (ab119526)

Overview

• Product nameGranzyme A Human ELISA Kit (with color giving dyes)
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Overall				8.7%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Overall				8.6%

• Tests
  1 x 96 test
• Sample type
  Cell culture supernatant, Serum, Plasma
• Assay typeSandwich
• Sensitivity
  0.4 pg/ml
• Range
  0.8 pg/ml - 600 pg/ml
• Recovery

  78 %
  

• Species reactivity
  Reacts with: Human
  
• Product overview

  ab119526 Granzyme A Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of Human Granzyme A. ab119526 is for research use only and is not for diagnostic or therapeutic procedures.

  Granzymes are exogenous serine proteinases that are released from cytoplasmic granules of cytotoxic lymphocytes (CTLs) and NK cells. The name 'granzymes' is derived from: granules + enzymes. These granules contain next to granzymes other proteins including a poreforming protein (Perforin). Upon binding of the CTL to a target cell (by CTL-receptor and antigen-presenting MHC molecules on the target cell) the contents of the granules are released in the intercellular space where after perforine will perforate the target cell membrane by forming transmembrane pores. Through these pores the granzymes can now enter the cytosol of the target cell. Granzyme B activates the intracellular cascade of caspases finally resulting in the killing of the target cells.

  Percentages of the Granzyme A and B positive CTLs can be determined by flow cytometry and immunocytochemical methods for many disorders.

  Not all granzymes enter the target cell, part of them also 'leak' in to the peripheral blood and other biological fluids. Detectable amounts of granzymes have been found to circulate in healthy volunteers. These soluble granzymes can be measured by ELISAs. Increased levels of soluble granzymes have been found with patients suspected of an increased NK cell and CTL-response caused by systemic viral infections such as EBV, HIV, CMV, hepatitis A and Dengue fever. Soluble Granzyme A and B is increased in synovial fluid from rheumatoid arthritis and significantly higher than levels in patients with osteoarthrosis. Granzymes are likely involved in the acute rejection of kidney-transplants, as infiltrating lymphocytes in the rejected kidney strongly express granzymes. Increasing plasma levels of soluble granzymes in patients with a kidney transplants.

  In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

• Tested applicationsSandwich ELISA more details

Properties

• Storage instructionsPlease refer to protocols.

• Components	1 x 96 tests
  Adhesive Films	4 units
  Biotin-Conjugate anti-human Granzyme A monoclonal antibody	2 x 70µl
  Blue-Dye	1 x 0.4ml
  Dilution Buffer Concentrate (5x)	1 x 7ml
  Green-Dye	1 x 0.4ml
  Human Granzyme A Standard	2 vials
  Monoclonal anti-human Granzyme A-coated microwell plate	1 unit
  Red-Dye	1 x 0.4ml
  Stop Solution	1 x 15ml
  Streptavidin-HRP	1 x 150µl
  Substrate Solution	1 x 15ml
  Wash Buffer Concentrate (20x)	1 x 50ml

• Research Areas
  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Granzyme and perforins ELISA kits

  • Immunology
  • Innate Immunity
  • NK Cells

  • Immunology
  • Adaptive Immunity
  • T Cells
  • Cytotoxic Cells

  • Cell Biology
  • Apoptosis
  • Extracellular Signals
  • Granzymes

Applications

Granzyme A Human ELISA Kit (with color giving dyes) images

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  Sandwich ELISA - Granzyme A Human ELISA Kit (with color giving dyes) (ab119526)
  Representative standard curve using ab119526.

Protocols

Protocol Booklet