Granzyme B Human ELISA Kit (ab46133)

Overview

• Product nameGranzyme B Human ELISA KitSee all Granzyme B kits ...
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  A	15	356pg/ml	18	5.06%
  B	15	73pg/ml	5	7.38%

  Inter-assay

  Sample	n	Mean	SD	CV%
  A	8	536pg/ml	30	5.59%

• Tests
  2 x 96 well plate
• Sample type
  Cell culture supernatant, Serum, Plasma
• Assay typeSandwich
• Sensitivity
  < 20 pg/ml
• Range
  31.25 pg/ml - 1000 pg/ml
• Recovery

  Sample specific recovery

  Sample type	Average %	Range
  Serum	73	31.25pg/ml - 1000pg/ml

• Assay time
  3h 00m
• Species reactivity
  Reacts with: Human
  
• Product overview

  The Human Granzyme B ELISA is to be used for the in-vitro quantitative determination of Granzyme B in human serum, plasma, buffered solutions or cell culture medium. This kit has been configured for research use only and is not to be used in diagnostic procedures.

  Principal:

  The Granzyme B Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for Granzyme B has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Granzyme B concentrations and unknowns are pipetted into these wells. During the first incubation, the Granzyme B antigen and a biotinylated monoclonal antibody specific for Granzyme B are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Granzyme B present in the samples.

   

  No cross reactivity with TRAIL, TNF alpha, Interferon gamma, Fas, IL1 alpha, IL4, IL6, IL8, IL13
  
  Range: 31.25 pg/ml - 1000 pg/ml
  
  Sensitivity < 20 pg/ml
  

• Notes

  Safety Notes:

  • For research use only.
  • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.
  • Avoid any skin contact with H₂SO₄ and TMB. In case of contact, wash thoroughly with water.
  • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
  • Do not pipette by mouth.
  • The stop solution in this kit contains 9.8% sulphuric acid.

• Tested applicationsELISA more details

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	Identifier	2 x 96 tests
  12 strips		8 units
  Biotinylated Antibody Diluent	Red	1 x 13ml
  Biotinylated anti-Granzyme B	Red	2 x 0.4ml
  Chromogen TMB		1 x 24ml
  H2SO4: Stop Reagent	Black	2 x 11ml
  HRP Diluent	Red	1 x 23ml
  Standard	Yellow	4 vials
  Standard Diluent: Buffer.x10 Concentrated	Black	1 x 25ml
  Streptavidin-HRP		4 x 5µl
  Washing Buffer.x200 Concentrated	White	2 x 10ml

• Research Areas
  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Granzyme and perforins ELISA kits

  • Immunology
  • Adaptive Immunity
  • T Cells
  • Cytotoxic Cells

  • Cell Biology
  • Apoptosis
  • Extracellular Signals
  • Granzymes

Applications

Granzyme B Human ELISA Kit images

• 

  ELISA - Human Granzyme B ELISA Kit (ab46133)
  Typical Granzyme B standard curve ranging from 31.25 to 1000 pg/mL.

Protocols

• Human Granzyme B ELISA kit