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Overview

  • Product nameGranzyme B Human ELISA KitSee all Granzyme B kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 15 356pg/ml 18 5.06%
    B 15 73pg/ml 5 7.38%
    Inter-assay
    Sample n Mean SD CV%
    A 8 536pg/ml 30 5.59%
    B 8 138pg/ml 9 6.76%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 20 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 73 31.25pg/ml - 1000pg/ml

  • Assay time
    3h 45m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Granzyme B Human ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement Human serum, plasma, buffered solutions or cell culture media.

     

     

     

    PRINCIPLE OF THE METHOD

     

    The Granzyme B Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for Granzyme B has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Granzyme B concentrations and unknowns are pipetted into these wells.

    During the first incubation, the Granzyme B antigen and a biotinylated monoclonal antibody specific for Granzyme B are simultaneously incubated.

    After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Granzyme B present in the samples.

     

     

    No cross reactivity with TRAIL, TNF alpha, Interferon gamma, Fas, IL1 alpha, IL4, IL6, IL8, IL13

  • Notes

    Safety Information:

    • For research use only.

     

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

     

     

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

     

     

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

     

     

    • Do not pipette by mouth.

     

     

    • The stop solution in this kit contains 9.8% sulphuric acid.

     

  • Tested applicationsELISA more details

Properties

  • RelevanceThe lytic enzymes of cytotoxic lymphocytes seem to be contained within lysosome-like granules found in the cytoplasm. These granules contain perforin, a membrane pore-forming protein, and a family of serine proteases (granzymes). Granzyme B, a 32 kDa protein, is a serine Aspase associated with the granules of Natural Killer (NK) cells. Granzyme B seems to be able to elicit cell death, perhaps by activating an endogenous pathway of programmed cell death.
  • Cellular localizationCytoplasmic
    • Alternative names
        C11Cathepsin G like 1CTLA 1
        CTSGL1Cytotoxic T lymphocyte proteinase 2EC 3.4.21.79Fragmentin 2Granzyme 2HLPHuman lymphocyte proteinLymphocyte proteaseSECTT cell serine protease 1 3E
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab46142 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Granzyme B Human ELISA Kit images

      • ELISA - Human Granzyme B ELISA kit (ab46142)
        ELISA - Human Granzyme B ELISA kit (ab46142)
        Typical Granzyme B standard curve ranging from 1000 to 31.25 pg/mL

      References for Granzyme B Human ELISA Kit (ab46142)

      This product has been referenced in:
      • Patel SA  et al. Mesenchymal stem cells protect breast cancer cells through regulatory T cells: role of mesenchymal stem cell-derived TGF-beta. J Immunol 184:5885-94 (2010). Human . Read more (PubMed: 20382885) »
      • Prakash MD  et al. Active and zymogen forms of granzyme B are constitutively released from cytotoxic lymphocytes in the absence of target cell engagement. Immunol Cell Biol 87:249-54 (2009). Human . Read more (PubMed: 19079360) »

      See all 3 Publications for this product

      Publishing research using ab46142 ? Please let us know so that we can cite the reference in this datasheet

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      What are the species of the capture and detection antibodies? What is the concentration of the detection antibody?

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      Antibodies included in the Granzyme kit are raised in mouse. The capture antibody coated onto the wells is (Fab)' 2 IgG1, and the biotiinylated detection antibody tracer is whole IgG1, and final concentration is in ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"