The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/25. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/10 - 1/25.
1/100 - 1/250. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
1/100 - 1/250.
FunctionThis enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.
Cellular localizationCytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.