Overview
- Product nameAnti-Granzyme B antibody [GZB01]See all Granzyme B primary antibodies ...
- DescriptionMouse monoclonal [GZB01] to Granzyme B
- SpecificityThis antibody reacts with a 32 kD protein.
- Tested applicationsIHC-P, IHC-Fr, WB, IP more details
- Species reactivityReacts with: Human
- Immunogen
Recombinant full length protein (Human).
- Positive controlIHC: Hodgkin’s lymphoma. WB: human lymphoid cell extract
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, Tissue culture supernatant - PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberGZB01
- Myelomaunknown
- IsotypeIgG2a
- Light chain typeunknown
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab3654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: 1/10 - 1/25. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| IHC-Fr | IHC-Fr: 1/10 - 1/25. |
| WB | WB: 1/100 - 1/250. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
| IP | IP: 1/100 - 1/250. |
Target
- FunctionThis enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
- Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. - Cellular localizationCytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
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Database links
- Entrez Gene: 3002 Human
- Entrez Gene: 3002 Human
- Entrez Gene: 3002 Human
- Entrez Gene: 3002 Human
- Omim: 123910 Human
- SwissProt: P10144 Human
- SwissProt: P10144 Human
- SwissProt: P10144 Human
- SwissProt: P10144 Human
- Unigene: 1051 Human
see all
Target information above from: UniProt accession
P10144
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- C11 antibodyCathepsin G like 1 antibodyCathepsin G-like 1 antibody
- Cathespin G Like 1 antibodyCCPI antibodyCGL 1 antibodyCGL1 antibodyCSP B antibodyCSPB antibodyCTLA 1 antibodyCTLA-1 antibodyCTLA1 antibodyCTSGL 1 antibodyCTSGL1 antibodyCytotoxic serine protease B antibodyCytotoxic T Lymphocyte Associated Serine Esterase 1 antibodyCytotoxic T lymphocyte proteinase 2 antibodyCytotoxic T-lymphocyte proteinase 2 antibodyFragmentin 2 antibodyFragmentin-2 antibodyFragmentin2 antibodyGRAB_HUMAN antibodyGranzyme 2 antibodyGranzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) antibodyGranzyme B antibodyGranzyme-2 antibodyGranzyme2 antibodyGranzymeB antibodyGRB antibodyGZMB antibodyHLP antibodyHLP antibodyHuman lymphocyte protein antibodyLymphocyte protease antibodyProtease Serine B antibodySECT antibodyT cell serine protease 1 3E antibodyT cell serine protease 1-3E antibodyT-cell serine protease 1-3E antibody
see all
Anti-Granzyme B antibody [GZB01] images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Granzyme B antibody [GZB01] (ab3654)](http://a.abcam.com/ps/datasheet/Images/3/ab3654/ab3654_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Granzyme B antibody [GZB01] (ab3654)ab3654 - immunohistochemistry
Formalin fixed paraffin embedded human Hodgkin's lymphoma with EBV stained with Granzyme B using ABC and AEC chromagen.
References for Anti-Granzyme B antibody [GZB01] (ab3654)
ab3654 has not yet been referenced specifically in any publications.
