Growth Hormone Human ELISA kit (ab100526)
- Product nameGrowth Hormone Human ELISA kitSee all Human Growth Hormone kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 4 pg/ml
- Range2.5 pg/ml - 600 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 94.25 94.25% - 104% Serum 92.17 77% - 102% Plasma 93.35 78% - 102%
- Species reactivityReacts with: Human
- Product overview
Abcam’s Human Growth Hormone ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Growth Hormone in serum, plasma, cell culture supernatants and urine.
This assay employs an antibody specific for human Growth Hormone coated on a 96-well plate. Standards and samples are pipette into the wells and Growth Hormone present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Growth Hormone antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Growth Hormone bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml 5x Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-human Growth Hormone Antibody 2 vials Growth Hormone Microplate 1 unit Growth Hormone Standard Human Recombinant 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
- GHNGrowth hormoneGrowth hormone 1HGHPituitary growth hormoneSOMA_HUMANSomatotropin
Our Abpromise guarantee covers the use of ab100526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
TYPICAL STANDARD CURVE - Assay Diluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Growth Hormone Human ELISA kit images
TYPICAL STANDARD CURVE - Assay DIluent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
References for Growth Hormone Human ELISA kit (ab100526)
ab100526 has not yet been referenced specifically in any publications.