You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameGrowth Hormone Human ELISA kitSee all Human Growth Hormone kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 4 pg/ml
  • Range
    2.5 pg/ml - 600 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.25 94.25% - 104%
    Serum 92.17 77% - 102%
    Plasma 93.35 78% - 102%

  • Species reactivity
    Reacts with: Human
  • Product overview

     

    Abcam’s Human Growth Hormone ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Growth Hormone in serum, plasma, cell culture supernatants and urine.

    This assay employs an antibody specific for human Growth Hormone coated on a 96-well plate. Standards and samples are pipette into the wells and Growth Hormone present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Growth Hormone antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Growth Hormone bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsELISA more details

Properties

  • FunctionPlays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • Involvement in diseaseDefects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
    Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
  • Sequence similaritiesBelongs to the somatotropin/prolactin family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P01241 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        gHGH-NGH1
        GHNGrowth hormoneGrowth hormone 1HGHPituitary growth hormoneSOMA_HUMANSomatotropin
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100526 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA.

      TYPICAL STANDARD CURVE - Assay Diluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

      Growth Hormone Human ELISA kit images

      • TYPICAL STANDARD CURVE – Assay Diluent B
        TYPICAL STANDARD CURVE – Assay Diluent B

        TYPICAL STANDARD CURVE - Assay DIluent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

      • TYPICAL STANDARD CURVE – Assay Diluent A
        TYPICAL STANDARD CURVE – Assay Diluent A

      Protocols

      References for Growth Hormone Human ELISA kit (ab100526)

      ab100526 has not yet been referenced specifically in any publications.

      Publishing research using ab100526 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100526 Submit a Question for ab100526

      Displaying 1 - 4 of 4 results for Abreviews and Q&A

      10
      Votes: Highest First

      I can find that kit contents consist of Assay diluent A and Assay diluent B.
      Assay diluent A is used for serum and plasma, Assay diluent B is used for cell culture.

      My sample is serum and can use Assay diluent A as your contents.
      But ...

      Read More

      Assay diluent A (serum) is required for diluting standards and samples if your experimental samples are serum. The other reagents (detection antibody and HRP-streptavidin) can be diluted in 1X diluent B, which is a buffer.

      Use Assay diluent B (...

      Read More

      I see a precipitate in assay diluent B. What should I do?

      Thank you for your email. As Assay Diluent B contains proteins and other solutes it would not be abnormal to see a little cloudiness or precipitation, especially if stored at a low temperature and/or for a long period of time. Most of the time bringing th...

      Read More

      Product code: 100526

      Inquiry: Hi, We are considering ordering this product. I've read the instructions for use and I have a question in regards to the usage of item F. As seen in Preparation of Reagents step 6 I have to add 100 ul of 1x Assay D...

      Read More

      Thank you for your enquiry.

      The reason the protocol for ab100526 Growth Hormone Human ELISA kit states storing the antibody concentrate at 4°C for only a few days is to avoid freeze-thaw cycles which can degrade the antibody. Storing the antibo...

      Read More

      Dear Technical   As you don't have  a file for Kit complaint the customer filled in only the needed details 1) Abcam product code  ab  Ab100526 2) Abcam order reference number or product batch number GR 6201-1 [526-8-8-W   C2] 3) Description o...

      Read More

      Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this kit. The details you have kindly provided will enable us to investigate this case for you and t...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"