Anti-Guanylate Cyclase antibody (ab53084)
Key features and details
- Rabbit polyclonal to Guanylate Cyclase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Guanylate Cyclase antibody -
Description
Rabbit polyclonal to Guanylate Cyclase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide derived from Human Guanylate Cyclase ß
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Positive control
- HuvEc cell extract; Paraffin-embedded human lung carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/300 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/300 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Relevance
Guanylate Cyclase is an heterodimer of an alpha and a beta chain. Its enzymatic activity transforms GTP to GMP releasing a diphosphate. It binds 1 or 2 hemes per heterodimer. It is activated by nitric oxide in the presence of magnesium or manganese ions. There are two types of guanylate cyclase: soluble form and membrane-associated receptor form. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 2974 Human
- Entrez Gene: 239134 Mouse
- Entrez Gene: 25206 Rat
- Omim: 603695 Human
- SwissProt: O75343 Human
- SwissProt: Q6XE41 Mouse
- SwissProt: P22717 Rat
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Alternative names
- GC SB2 antibody
- GCS beta 2 antibody
- Guanylate cyclase 1 soluble beta 2 antibody
- GUCY1B2 antibody
Images
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All lanes : Anti-Guanylate Cyclase antibody (ab53084) at 1/300 dilution
Lane 1 : HuvEc cell extract
Lane 2 : HuvEc cell extract with peptide
Predicted band size: 70 kDa
Observed band size: 70 kDa -
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Guanylate Cyclase ß antibody, ab53084, at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab53084 has been referenced in 1 publication.
- Wu RY & Ma N Expression of nitric oxide synthase and guanylate cyclase in the human ciliary body and trabecular meshwork. Chin Med J (Engl) 125:129-33 (2012). WB, IHC-P ; Human . PubMed: 22340479