Guinea pig IgG secondary antibody - H&L (ab6567)

Product Name 

Guinea pig IgG secondary antibody - H&L

Product type 

Secondary antibodies

Description 

Goat polyclonal to Guinea pig IgG - H&L (Cy5 ®)

Immunogen 

Guinea pig IgG, whole molecule (H&L)

Species Reactivity 

Minimum reactivity to Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-Fr, IHC-P

Application notes 
(see key)

Recommended dilutions
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Please refer to the relevant conjugation for dilutions specific for this product:

Cellular localization 

Secreted

Research areas 

Secondary antibodies >> anti-Guinea pig >> IgG >> Fluorophore >> Cy5
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Relevance 

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants.

This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

Raised in 

Goat

Clonality 

Polyclonal

Purity 

Immunogen affinity purified

Conjugation notes 

Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons)
Absorption Wavelength: 650 nm
Emission Wavelength: 667 nm
Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG

Storage buffer 

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS

Purification notes 

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.

Form 

Liquid

Conjugation 

Cy5 ®

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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