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Product Name
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Guinea pig IgG secondary antibody - H&L
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Product type
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Secondary antibodies
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Description
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Rabbit polyclonal to Guinea pig IgG - H&L (AP)
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Immunogen
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guinea pig IgG whole molecule
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Tested applications
(see key)
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Dot, ELISA, IHC-Fr, IHC-P, IM, WB
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Application notes
(see key)
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Recommended dilutions
suitable for immunoblotting (western or dot blot)
ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Cellular localization
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Secreted
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Research areas
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Secondary antibodies >> anti-Guinea pig >> IgG >> Enzyme >> Alkaline Phos
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Relevance
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Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Purity
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IgG fraction
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Conjugation notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using guinea pig IgG coupled to agarose beads
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Storage buffer
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0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
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Purification notes
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Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
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Form
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Liquid
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Conjugation
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Alkaline Phosphatase
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Guinea pig IgG secondary antibody - H&L is on this datasheet. But please do contact us if you would like any reassurance! |
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