Products:Epigenetics and Nuclear Signaling >> Histones >> Variants
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ab46666 |
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ab4626 has been referenced in 9 publications.
Publishing research using ab4626? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-H2AFZ antibody - ChIP Grade (ab4626) at 1 µg/ml + S.cerevisiae (Y190) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 14 kDa
Observed band size : 14 kDa
Exposure time : 20 minutes
Abcam recommends using 3% milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab4626 used in ChIP.Nuclear lysate prepared from S cerevisiae S288c was used.Cross linking (X-ChIP) was performed for 15 seconds with 1% formaldehyde.The detection step was performed using Real-time PCR.ab4626 was used at a 1/100 dilution for 4 hours at 4°C.
Image courtesy of Harsh Ja by Abreview.
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