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The H2A.X (pS139) and cleaved-PARP Human In-Cell ELISA Kit (IR) (ab131383) is designed to study the induction of DNA damage and/or apoptosis in response to various stimuli. Monoclonal antibodies specific to phospho-H2A.X (S139) and cleaved-PARP are used in the high-throughput duplexing plate-based assay. H2A.X is a histone H2A family member that is phosphorylated and recruited to sites of double-strand DNA breaks. Poly [ADP-ribose] polymerase 1 (PARP) is a DNA repair enzyme that is cleaved by activated caspases. Combined, these antibodies provide biomarkers of dsDNA breaks (pH2A.X) and apoptosis (cleaved-PARP).
Plates are available in our ICE (In-Cell ELISA) Support Pack (ab111542) which can be bought seperately.
|Components||1 x 96 tests|
|1000X IRDye-labeled Secondary Antibodies||1 x 24µl|
|100X Anti-cleaved-PARP Primary Antibody (Mouse Monoclonal)||1 x 120µl|
|100X Anti-H2A.X (pSer139) Rabbit Primary Antibody||1 x 120µl|
|100X Triton X-100||1 x 0.5ml|
|10X Blocking Buffer||1 x 10ml|
|10X Phosphate Buffered Saline||1 x 100ml|
|400X Tween-20||1 x 2ml|
|Janus Green Stain||1 x 17ml|
Our Abpromise guarantee covers the use of ab131383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|In-Cell ELISA||Use at an assay dependent concentration.|
ab131383 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"