H2A.X (pSer139) Human In-Cell ELISA Kit (ab131382)

Overview

• Product nameH2A.X (pSer139) Human In-Cell ELISA Kit
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  HeLa Cells				< 10%
• Tests
  1 x 96 well plate
• Sample type
  Adherent cells, Suspension cells
• Assay typeCell-based
• Species reactivity
  Reacts with: Human
  
• Product overview

  The H2A.X phospho Ser139 Human In-Cell ELISA Kit (IR) (ab131382) is designed to study the induction of DNA damage in response to various stimuli. A rabbit monoclonal antibody specific to H2A.X phospho Ser139 is used in this high-throughput duplexing plate-based assay. H2A.X phospho Ser139 is a reliable readout for double-stranded DNA breaks.

• Tested applicationsIn-Cell ELISA more details

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	1 x 96 tests
  1000X IRDye®-Labeled Secondary Antibody	1 x 24µl
  100X Anti-H2A.X (pSer139) Rabbit Primary Antibody	1 x 120µl
  100X Triton X-100	1 x 0.5ml
  10X Blocking Buffer	1 x 10ml
  10X Phosphate Buffered Saline	1 x 100ml
  400X Tween-20	1 x 2ml
  Janus Green Stain	1 x 17ml

• Research Areas
  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • In Cell ELISA Kits
  • Phosphoprotein ICE kits

  • Epigenetics and Nuclear Signaling
  • Histones
  • H2A
  • Phosphorylated

  • Epigenetics and Nuclear Signaling
  • Histones
  • H2A
  • Variants

Applications

H2A.X (pSer139) Human In-Cell ELISA Kit images

• 

  In-Cell ELISA - H2A.X pSer139 In Cell ELISA Kit (IR) (ab131382)
  Sample experiment using ab131382 on HeLa cells following drug treatment: H2A.X (pSer139) readout. HeLa cells were treated for 4 hours with dose titrations of Camptothecin, Etoposide and Staurosporin. The dashed grey line indicates the vehicle control signal.
• 

  Immunocytochemistry/ Immunofluorescence - H2A.X pSer139 In Cell ELISA Kit (IR) (ab131382)
  Specificity of H2A.X (pSer139) antibodies demonstrated by immunocytochemistry. The primary antibody used in this assay kit was validated by staining HeLa cells treated with 10 µM Camptothecin or vehicle for 4 hours and imaged by fluorescent microscopy. Note the absence of H2A.X (pSer139) in the untreated cells.
• 

  Western blot - H2A.X pSer139 In Cell ELISA Kit (IR) (ab131382)
  All lanes : H2A.X (pSer139) Human In-Cell ELISA Kit (ab131382)
  
  Lane 1 : Untreated cells
  Lane 2 : Camptothecin treated cells
  Lane 3 : UV exposed cells
  
• 

  Western blot - H2A.X pSer139 In Cell ELISA Kit (IR) (ab131382)
  Antibody specificity demonstrated by Western Blot Analysis: H2A.X (pSer139) is phospho-specific. Jurkat cells were stimulated with UV light exposure to induce H2A.X phospho Ser139 and then the UV treated lysate was treated with lambda protein phosphatase. Top panel: H2A.X (pSer139) is induced by UV treatment and the western blot band is sensitive to phosphatase treatment. Lower panel: In contrast, total H2A.X (ab124781) levels are not sensitive to phosphatase treatment.

Protocols

Protocol Booklet