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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab72183 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - H6PD antibody (ab72183)
Anti-H6PD antibody (ab72183) at 1 µg/ml +
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 88 kDa
Observed band size : 94 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes
Human GDH/6PGL endoplasmic bifunctional protein precursor (H6PD) has a predicted molecular weight of 88 kDa, however it is glycosylated and phosphorylated which may explain the bands appearing at a higher molecular weight (94 kDa)(SwissProt data).
Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Immunocytochemistry/ Immunofluorescence - H6PD antibody (ab72183)
ICC/IF image of ab72183 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72183, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2, and MCF-7 cells at 5µg/ml.
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