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Products:Signal Transduction >> Growth Factors/Hormones >> Hormones
Overview
Product name
Anti-H6PD antibody
See all H6PD products (4) ...
Description
Rabbit polyclonal to H6PD
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human H6PD.
(Peptide available as ab90254.)
Positive control
This antibody gave a positive signal in human liver tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Growth Factors/Hormones >> Hormones
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 94 kDa (predicted molecular weight: 88 kDa).
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.
Tissue specificity
Present in most tissues examined, strongest in liver.
Involvement in disease
Defects in H6PD are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
Sequence similarities
In the N-terminal section; belongs to the glucose-6-phosphate dehydrogenase family.
In the C-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.
Cellular localization
Endoplasmic reticulum lumen. Microsomes, endoplasmic reticulum lumen.
Target information above from: UniProt accessionO95479
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 6 phosphogluconolactonase antibody
- 6-phosphogluconolactonase antibody
- 6PGL antibody
see all
Database links
- Entrez Gene: 25796 Human
- Entrez Gene: 9563 Human
- Entrez Gene: 100198 Mouse
- Entrez Gene: 66171 Mouse
- Omim: 138090 Human
- SwissProt: O95336 Human
- SwissProt: O95479 Human
- SwissProt: Q8CFX1 Mouse
see all
Anti-H6PD antibody images:
Western blot - H6PD antibody (ab72183)
Anti-H6PD antibody (ab72183) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 88 kDa
Observed band size : 94 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes
Human GDH/6PGL endoplasmic bifunctional protein precursor (H6PD) has a predicted molecular weight of 88 kDa, however it is glycosylated and phosphorylated which may explain the bands appearing at a higher molecular weight (94 kDa)(SwissProt data).
Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Immunocytochemistry/ Immunofluorescence - H6PD antibody (ab72183)
ICC/IF image of ab72183 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72183, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2, and MCF-7 cells at 5µg/ml.
References for Anti-H6PD antibody (ab72183)
ab72183 has not yet been referenced specifically in any publications.
Publishing research using ab72183? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"