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Western blot - H6PD antibody (ab72183)
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Immunocytochemistry/ Immunofluorescence - H6PD antibody (ab72183)
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Product Name
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H6PD antibody
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See all H6PD antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to H6PD
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human H6PD.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 88 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in human liver tissue lysate.
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Cellular localization
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Endoplasmic reticulum
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Research areas
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Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Growth Factors/Hormones >> Hormones
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Relevance
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H6PD (hexose-6-phosphate dehydrogenase) oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates. Defects in H6PD are a cause of cortisone reductase deficiency, in which the activation of cortisone to cortisol does not occur, resulting in a phenotype resembling polycystic ovary syndrome (PCOS). H6PD interacts with 11 beta-hydroxysteroid dehydrogenase type I, and both proteins may play important roles in the pathogenesis of obesity and metabolic syndrome.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.20 - 0.40 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this H6PD antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for H6PD antibody images, references, products related to ab72183 and other tools.
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H6PD antibody images:
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 Western blot - H6PD antibody (ab72183)
H6PD antibody (ab72183) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 88 kDa
Observed band size : 94 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes
Human GDH/6PGL endoplasmic bifunctional protein precursor (H6PD) has a predicted molecular weight of 88 kDa, however it is glycosylated and phosphorylated which may explain the bands appearing at a higher molecular weight (94 kDa)(SwissProt data).
Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Immunocytochemistry/ Immunofluorescence - H6PD antibody (ab72183)
ICC/IF image of ab72183 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72183, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2, and MCF-7 cells at 5µg/ml.
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Search PubMed (MEDLINE) for references to H6PD
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H6PD antibody - more information
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Customer reviews (feedback) regarding H6PD antibody |
Customer FAQs regarding H6PD antibody |
Protocols for H6PD antibody |
Price and availability of products related to H6PD antibody |
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H6PD antibody - related products:
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H6PD antibody - other tools:
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Contact Abcam with a Technical Enquiry about H6PD antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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