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Anti-HA tag antibody [12CA5] (ab16918)

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Overview

Product name

Anti-HA tag antibody [12CA5]
See all HA tag products (36) ...

Description

Mouse monoclonal [12CA5] to HA tag

Tested applications

ELISA, WB, IP, ICC/IFmore details

Immunogen

Balb/C mice immunized with recombinant influenza virus hemagglutinin.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.08% Sodium Azide
Constituents: 50% Glycerol, PBS

Concentration

Concentration information loading...

Purity

>95% by SDS-PAGE

Purification notes

The antibody was purified to >95% homogeneity by standard chromatographic techniques.

Clonality

Monoclonal

Clone number

12CA5

Isotype

IgG2b

Research areas

Tags & Cell Markers >> Epitope Tags >> HA Tag

  • Western blot - HA tag antibody [12CA5] (ab16918)Western blot - HA tag antibody [12CA5] (ab16918) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab16918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/1000

    ELISA: 1/1000

  • ShowHide1 Image

    WB

     WB: 1/1000(Initially 1h ...Read more →

    WB: 1/1000(Initially 1h incubation at RT is recommended.)

  • ShowHide

    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

  • ShowHide

    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent dilution. (PubMed: 19442237)

Target

Relevance

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Cellular localization

Not Applicable

Alternative names

  • HA epitope tag antibody
  • hemagglutinin antibody

Anti-HA tag antibody [12CA5] images:

  Western blot - HA tag antibody [12CA5] (ab16918)

Western blot - HA tag antibody [12CA5] (ab16918)

Western blot using ab16918 at 1:1000 dilution.
Lane 1: HEK293 cells
Lane 2: HEK293 cells expressing HA-tagged Rem protein

References for Anti-HA tag antibody [12CA5] (ab16918)

This product has been referenced in:

  • Avunie-Masala Ret al. Phospho-regulation of kinesin-5 during anaphase spindle elongation. J Cell Sci 124:873-8 (2011).Read more (PubMed: 21378308) »
  • Kulkarni AS & Fortunato EA Stimulation of homology-directed repair at I-SceI-induced DNA breaks during the permissive life cycle of human cytomegalovirus. J Virol 85:6049-54 (2011).Read more (PubMed: 21490102) »

See all 18 publications for this product

Publishing research using ab16918? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"