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Immunocytochemistry/ Immunofluorescence - HA tag antibody [4C12] - ChIP Grade (ab1424)
![Immunocytochemistry/ Immunofluorescence - HA tag antibody [4C12] - ChIP Grade (ab1424)](/ps/datasheet/images/ab1424_1.jpg)
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ChIP - HA tag antibody [4C12] (ab1424)
![ChIP - HA tag antibody [4C12] (ab1424)](/ps/datasheet/images/ab1424_2.jpg)
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Product Name
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HA tag antibody [4C12] - ChIP Grade
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See all HA tag antibodies (27)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [4C12] to HA tag - ChIP Grade
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Specificity
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The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells.
The antibody works in ChIP but less well than ab9110 (see ChIP data).
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Tested applications
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ChIP, ELISA, ICC, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use 3 µg per 25 µg chromatin (see figure legend).
ICC: Use at a concentration of 10 µg/ml.
IP: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 1 µg/ml.
ELISA: Suggested working dilution of 1/1000 (see PMID: 17167031).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Tags & Cell Markers >> Epitope Tags >> HA Tag
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Relevance
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Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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4C12
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Isotype
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IgG1
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Storage buffer
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PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HA tag antibody [4C12] - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HA tag antibody [4C12] - ChIP Grade images, references, products related to ab1424 and other tools.
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HA tag antibody [4C12] - ChIP Grade images:
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 Immunocytochemistry/ Immunofluorescence - HA tag antibody [4C12] - ChIP Grade (ab1424)
This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunoflourescence was performed. Dilution of 1:200 was used and incubated for 2hrs with antibody. This image was kindly supplied as part of the review submitted by one of our customers.
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ChIP - HA tag antibody [4C12] (ab1424)
Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 3µg of ab1424 (anti-HA, light blue) and 3µg of ab18337 (anti-BORIS, dark blue), and 20µl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
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References for HA tag antibody [4C12] - ChIP Grade (ab1424)
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This product has been used in: (two most recent references)
Hoe HS et al. Fyn Modulation of Dab1 Effects on Amyloid Precursor Protein and ApoE Receptor 2 Processing. J Biol Chem 283:6288-99 (2008). IP. PubMed: 18089558
Frauli M et al. Amino-pyrrolidine tricarboxylic acids give new insight into group III metabotropic glutamate receptor activation mechanism. Mol Pharmacol 71:704-12 (2007). PubMed: 17167031
See all 9 publication references for this product.
If you publish research using ab1424 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to HA tag
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HA tag antibody [4C12] - ChIP Grade - more information
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Customer reviews (feedback) regarding HA tag antibody [4C12] - ChIP Grade |
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Price and availability of products related to HA tag antibody [4C12] - ChIP Grade |
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HA tag antibody [4C12] - ChIP Grade - related products:
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HA tag antibody [4C12] - ChIP Grade - other tools:
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Contact Abcam with a Technical Enquiry about HA tag antibody [4C12] - ChIP Grade |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - HA tag antibody [4C12] - ChIP Grade (ab1424)](/index.html?datasheet=1424#ab1424_1.jpg)
![ChIP - HA tag antibody [4C12] (ab1424)](/index.html?datasheet=1424#ab1424_2.jpg)
