![Immunocytochemistry/ Immunofluorescence - HA tag antibody [4C12] - ChIP Grade (ab1424) image](#ab1424_1.jpg)
![ChIP - HA tag antibody [4C12] (ab1424) image](#ab1424_2.jpg)
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Products:Tags & Cell Markers >> Epitope Tags >> HA Tag
Overview
Product name
Anti-HA tag antibody [4C12] - ChIP Grade
See all HA tag products (36) ...
Description
Mouse monoclonal [4C12] to HA tag - ChIP Grade
Specificity
The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells. The antibody works in ChIP but less well than ab9110 (see ChIP data).
Tested applications
ELISA, ChIP, ICC, IP, WBmore details
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
4C12
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Tags & Cell Markers >> Epitope Tags >> HA Tag
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/1000(PubMed: 17167031)
ChIP: Use a concentration of 3 µg/ml(Use 3 µg per 25 µg chromatin (see figure legend). )
ICC: Use a concentration of 10 µg/ml
IP: Use a concentration of 10 µg/ml
WB: Use a concentration of 1 µg/ml
Target
Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Cellular localization
Not Applicable
Alternative names
- HA epitope tag antibody
- hemagglutinin antibody
Anti-HA tag antibody [4C12] - ChIP Grade images:
Immunocytochemistry/ Immunofluorescence - HA tag antibody [4C12] - ChIP Grade (ab1424)
![Immunocytochemistry/ Immunofluorescence - HA tag antibody [4C12] - ChIP Grade (ab1424)](/ps/datasheet/Images/1/ab1424/ab1424_1.jpg)
This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunoflourescence was performed. Dilution of 1:200 was used and incubated for 2hrs with antibody.
This image was kindly supplied as part of the review submitted by one of our customers.
ChIP - HA tag antibody [4C12] (ab1424)
![ChIP - HA tag antibody [4C12] (ab1424)](/ps/datasheet/Images/1/ab1424/ab1424_2.jpg)
Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 3µg of ab1424 (anti-HA, light blue) and 3µg of ab18337 (anti-BORIS, dark blue), and 20µl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
References for Anti-HA tag antibody [4C12] - ChIP Grade (ab1424)
This product has been referenced in:
- Narayanan MSet al. NLP is a novel transcription regulator involved in VSG expression site control in Trypanosoma brucei. Nucleic Acids Res 39:2018-31 (2011). ChIP.Read more (PubMed: 21076155) »
- Hoe HSet al. Fyn Modulation of Dab1 Effects on Amyloid Precursor Protein and ApoE Receptor 2 Processing. J Biol Chem 283:6288-99 (2008). IP.Read more (PubMed: 18089558) »
See all 9 publications for this product
Publishing research using ab1424? Please let us know so that we can cite the reference in this datasheet
![HA tag antibody [4C12] - ChIP Grade for Western blot in Human (1424)](/ps/datasheet/images/1/ab1424/HA-tag-Primary-antibodies-ab1424-1.gif)
![HA tag antibody [4C12] - ChIP Grade for IP in Human (1424)](/ps/Reviews/Images/ab4334_13420.jpg)
![HA tag antibody [4C12] - ChIP Grade for WB in Human (1424)](/ps/Reviews/Images/ab6483_24187.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"