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Anti-HA tag antibody [4C12] (FITC) (ab1789)

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Overview

Product name

Anti-HA tag antibody [4C12] (FITC)
See all HA tag products (39) ...

Description

Mouse monoclonal [4C12] to HA tag (FITC)

Conjugation

FITC

Specificity

The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells.

Tested applications

Flow Cyt, IFmore details

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

4C12

Isotype

IgG1

Research areas

Tags & Cell Markers >> Epitope Tags >> HA Tag

Applications

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Our Abpromise guarantee covers the use of ab1789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Flow Cyt: Use at an assay dependent dilution (PMID 14647427).
IF: Use at a concentration of 2 - 10 µg/ml.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Alternative names

  • HA epitope tag antibody
  • hemagglutinin antibody

References for Anti-HA tag antibody [4C12] (FITC) (ab1789)

This product has been referenced in:

  • Yamashiro H  et al. Stimulation of human butyrophilin 3 molecules results in negative regulation of cellular immunity. J Leukoc Biol : (2010). Flow Cyt.Read more (PubMed: 20610803) »
  • Liu WM  et al. Enhancing the in vitro cytotoxic activity of Delta9-tetrahydrocannabinol in leukemic cells through a combinatorial approach. Leuk Lymphoma 49:1800-9 (2008).Read more (PubMed: 18608861) »

See all 4 publications for this product

Publishing research using ab1789? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"