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ab46666 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
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Dear xxxxxx, |
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ANSWER: |
That is no problem. I have arranged for a different vial to be sent directly to you. This is on order number 1095224(FOCR 4502276). If you have any problems receiving it please do let me know. |
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One of our clients is experiencing problems with ab9110 Lot.# GR33978. They used it successfully multiple times before this time it didn't give any signal in their experiments. |
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ANSWER: |
Thank you for contacting us. |
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Product code: 9110 |
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ANSWER: |
Thank you for your reply. |
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Product code: 9110 |
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ANSWER: |
Thank you for contacting us and reporting the problems encountered with the anti-HA tag antibody (ab9110). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. |
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I think I have to try it because I need a antibody from rabbit. I have not found any antibody from rabbit that could be used in WB and eventually in IHC. So I would like to try it. |
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ANSWER: |
My suggestion would be ab9110 Anti-HA tag antibody - ChIP Grade under this circumstances: it has been used in many publications, and has 20 WB Abreviews and an excellent rating in total. However, most important, it can be used in a lot of different application, which implicates that the antibody is quite "robust" in detecting its target. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
This image was kindly supplied as part of the review submitted by Kasper Fugger. Immunofluorescence staining of U-2 cells expressing HA-tagged protein with ab9110.
Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 20µl of Protein A/G sepharose beads, and 3µg of ab9110 (anti-HA, light blue) or, 3µg of ab18337 (anti-Boris, dark blue). A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
ab 9110 at a 1/200 dilution staining the mouse olineu cell line (oligodendrocyte precursor cell) by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy5 conjugated goat anti-rabbit antibody.
This image is courtesy of an Abreview submitted by Katarina Trajkovic on 15 March 2006
All lanes : Anti-HA tag antibody - ChIP Grade (ab9110) at 1/4000 dilution
Lane 1 : 15ug untransfected wcl lysate
Lane 2 : 293FT cells transfected with 15kDa HA tagged Vpr (an HIV1 accessory protein)
Secondary
HRP conjugated Goat anti-Rabbit
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 15 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 seconds
This image is courtesy of an Abreview
ab9110 was diluted to 4 µg/mg lysate and incubated with a nuclear lysate of HEK293T cells transiently expressing HA-tagged protein and a Protein A matrix for 2 hours a 23°C to achieve immunoprecipitation. 1000 µg of lysate was present in the input.
A HRP-conjugated anti-rabbit HA monoclonal antibody diluted 1/1000 was used for the Western Blot step.
This image is courtesy of an anonymous Abreview
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