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Immunocytochemistry/ Immunofluorescence - HA tag antibody - ChIP Grade (ab9110)
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ChIP - HA tag antibody - ChIP Grade (ab9110)
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Immunocytochemistry/ Immunofluorescence - HA tag antibody - ChIP Grade (ab9110)
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Product Name
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HA tag antibody - ChIP Grade
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See all HA tag antibodies (27)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HA tag - ChIP Grade
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Immunogen
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YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH.
BLAST 'YPYDVPDYA' with  or 
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Specificity
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ELISA: The anti HA diluted 1:70.000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate.
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Tested applications
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ChIP, ChIP/Chip, ELISA, ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use 3µg for 25µg of chromatin
ChIP/Chip: Use at an assay dependent dilution.
ELISA: 1/200 - 1/500.
ICC/IF: Use at a concentration of 1 - 4 µg/ml.
IP: Use at an assay dependent dilution.
WB: 1/4000 - 1/10000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Tags & Cell Markers >> Epitope Tags >> HA Tag
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Relevance
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Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS with 0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HA tag antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HA tag antibody - ChIP Grade images, references, products related to ab9110 and other tools.
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HA tag antibody - ChIP Grade images:
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 Immunocytochemistry/ Immunofluorescence - HA tag antibody - ChIP Grade (ab9110)
This image was kindly supplied as part of the review submitted by Kasper Fugger. Immunofluorescence staining of U-2 cells expressing HA-tagged protein with ab9110.
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ChIP - HA tag antibody - ChIP Grade (ab9110)
Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 20µl of Protein A/G sepharose beads, and 3µg of ab9110 (anti-HA, light blue) or, 3µg of ab18337 (anti-Boris, dark blue). A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
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Immunocytochemistry/ Immunofluorescence - HA tag antibody - ChIP Grade (ab9110)
ab 9110 at a 1/200 dilution staining the mouse olineu cell line (oligodendrocyte precursor cell) by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy5 conjugated goat anti-rabbit antibody. This image is courtesy of an Abreview submitted by Katarina Trajkovic on 15 March 2006
See Abreview
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Western blot - HA tag antibody - ChIP Grade (ab9110)
All lanes : HA tag antibody - ChIP Grade (ab9110) at 1/4000 dilution
Lane 1 : 15ug untransfected wcl lysate
Lane 2 : 293FT cells transfected with 15kDa HA tagged Vpr (an HIV1 accessory protein)
Secondary
HRP conjugated Goat anti-Rabbit
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 15 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 seconds
This image is courtesy of an Abreview
See Abreview
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Immunoprecipitation - HA tag antibody - ChIP Grade (ab9110)
ab9110 was diluted to 4 µg/mg lysate and incubated with a nuclear lysate of HEK293T cells transiently expressing HA-tagged protein and a Protein A matrix for 2 hours a 23°C to achieve immunoprecipitation. 1000 µg of lysate was present in the input. A HRP-conjugated anti-rabbit HA monoclonal antibody diluted 1/1000 was used for the Western Blot step. This image is courtesy of an anonymous Abreview
See Abreview
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References for HA tag antibody - ChIP Grade (ab9110)
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This product has been used in: (two most recent references)
Noon AT et al. 53BP1-dependent robust localized KAP-1 phosphorylation is essential for heterochromatic DNA double-strand break repair. Nat Cell Biol 12:177-84 (2010). PubMed: 20081839
Chou YH et al. Diversity and wiring variability of olfactory local interneurons in the Drosophila antennal lobe. Nat Neurosci : (2010). PubMed: 20139975
See all 45 publication references for this product.
If you publish research using ab9110 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to HA tag
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