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Products:Tags & Cell Markers >> Epitope Tags >> HA Tag
Overview
Product name
Anti-HA tag antibody - ChIP Grade
See all HA tag products (36) ...
Description
Rabbit polyclonal to HA tag - ChIP Grade
Specificity
ELISA: The anti HA diluted 1:70.000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate.
Tested applications
ChIP/Chip, IP, ELISA, WB, ICC/IF, ICC, ChIPmore details
Immunogen
YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH.
YPYDVPDYA
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Tags & Cell Markers >> Epitope Tags >> HA Tag
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP/Chip
Five stars (1 Abreview)ELISA
- 1 Image
ChIP
Application notes
ChIP: Use 3µg for 25µg of chromatin
ChIP/Chip: Use at an assay dependent dilution.
ELISA: 1/200 - 1/500.
ICC/IF: Use at a concentration of 1 - 4 µg/ml.
IP: Use at an assay dependent dilution.
WB: 1/4000 - 1/10000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Cellular localization
Not Applicable
Alternative names
- HA epitope tag antibody
- hemagglutinin antibody
Anti-HA tag antibody - ChIP Grade images:
Immunocytochemistry/ Immunofluorescence - HA tag antibody - ChIP Grade (ab9110)
This image was kindly supplied as part of the review submitted by Kasper Fugger. Immunofluorescence staining of U-2 cells expressing HA-tagged protein with ab9110.
ChIP - HA tag antibody - ChIP Grade (ab9110)
Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 20µl of Protein A/G sepharose beads, and 3µg of ab9110 (anti-HA, light blue) or, 3µg of ab18337 (anti-Boris, dark blue). A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
Immunocytochemistry/ Immunofluorescence - HA tag antibody - ChIP Grade (ab9110)
ab 9110 at a 1/200 dilution staining the mouse olineu cell line (oligodendrocyte precursor cell) by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy5 conjugated goat anti-rabbit antibody.
This image is courtesy of an Abreview submitted by Katarina Trajkovic on 15 March 2006
Western blot - HA tag antibody - ChIP Grade (ab9110)
All lanes : Anti-HA tag antibody - ChIP Grade (ab9110) at 1/4000 dilution
Lane 1 : 15ug untransfected wcl lysate
Lane 2 : 293FT cells transfected with 15kDa HA tagged Vpr (an HIV1 accessory protein)
Secondary
HRP conjugated Goat anti-Rabbit
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 15 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 seconds
This image is courtesy of an Abreview
Immunoprecipitation - HA tag antibody - ChIP Grade (ab9110)
ab9110 was diluted to 4 µg/mg lysate and incubated with a nuclear lysate of HEK293T cells transiently expressing HA-tagged protein and a Protein A matrix for 2 hours a 23°C to achieve immunoprecipitation. 1000 µg of lysate was present in the input.
A HRP-conjugated anti-rabbit HA monoclonal antibody diluted 1/1000 was used for the Western Blot step.
This image is courtesy of an anonymous Abreview
References for Anti-HA tag antibody - ChIP Grade (ab9110)
This product has been referenced in:
- Narayanan MSet al. NLP is a novel transcription regulator involved in VSG expression site control in Trypanosoma brucei. Nucleic Acids Res 39:2018-31 (2011). WB, ICC/IF.Read more (PubMed: 21076155) »
- Lukashova Vet al. CK2 phosphorylation of an acidic Ser/Thr di-isoleucine motif in the Na+/H+ exchanger NHE5 isoform promotes association with beta-arrestin2 and endocytosis. J Biol Chem 286:11456-68 (2011).Read more (PubMed: 21296876) »
See all 56 publications for this product
Publishing research using ab9110? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"