Abcam guarantees this product to work in the species/application used in this Abreview.
Human Cell (HeLa)
Yes - 0.1% Triton X-100/0.02% SDS
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
3% paraformaldehyde, 0.02% glutaraldehyde
Other product details
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA, 0.1% Triton, 0.02% SDS in PBS
Non-Abcam antibody was used: goat anti rabbit IgG, Alexa Fluor 647 Host species: Goat Clonality: Polyclonal Conjugation: Alexa Fluor 647
The antibody works very well for the detection of cytoplasmic proteins by IF. The antibody produces a nuclear background, however, and is thus not suitable for the detection of low abundance nuclear proteins.
In the figure, a non-Abcam mouse anti-HA and ab9110 are used to detect a low-abundance HA-tagged nuclear envelope protein. Nuclear rim staining is clearly discernable with the mouse anti-HA antibody (upper panel). Nuclear rim staining of the protein of interest is barely recognizable with ab9110 because of a significant nuclear background produced by this antibody. This background is also visible in non-transfected cells (asterisks).
Abcam user community
Submitted Jan 25 2017
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