Western blot abreview for Anti-HA tag antibody - ChIP Grade

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells)
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Loading amount
15 µg
Specification
HEK293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/4000

Secondary antibody

Dilution
1/10000
Name

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 15 kDa
Username

Rebecca Fu

Verified customer

Submitted Apr 17 2018

Sign up