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ab18181 has been referenced in 7 publications.
Publishing research using ab18181? Please let us know so that we can cite the reference in this datasheet
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Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).
Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).
All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution
Lane 1 : WCE from cell line transfected for HA-tagged protein
Lane 2 : WCE from a cell line transfected with empty vector
Lysates/proteins at 50 µg per lane.
Secondary
HRP conjugated Goat anti-mouse IgG (H+L)
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.
This image is courtesy of an anonymous Abreview
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