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Western blot - HA tag antibody [HA.C5] (ab18181)
![Western blot - HA tag antibody [HA.C5] (ab18181)](/ps/datasheet/images/ab18181_1.jpg)
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Immunocytochemistry/ Immunofluorescence - HA tag antibody [HA.C5] (ab18181)
![Immunocytochemistry/ Immunofluorescence - HA tag antibody [HA.C5] (ab18181)](/ps/datasheet/images/ab18181_2.jpg)
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Western blot - HA tag antibody [HA.C5] (ab18181)
![Western blot - HA tag antibody [HA.C5] (ab18181)](/ps/datasheet/images/ab18181_3.jpg)
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Product Name
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HA tag antibody [HA.C5]
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See all HA tag antibodies (27)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [HA.C5] to HA tag
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Immunogen
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Synthetic peptide from influenza hemagglutinin epitope: YPYDVPDYA conjugated to KLH.
BLAST 'YPYDVPDYA' with  or 
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Reacts with
(species key)
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N/A
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Specificity
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Recognizes HA-tagged proteins overexpressed in cells, including both amino- and carboxy-termini of targeted proteins in transfected mammalian cells.
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/200.
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/1000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Tags & Cell Markers >> Epitope Tags >> HA Tag
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Relevance
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Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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HA.C5
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Isotype
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IgG3
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HA tag antibody [HA.C5] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HA tag antibody [HA.C5] images, references, products related to ab18181 and other tools.
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HA tag antibody [HA.C5] images:
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 Western blot - HA tag antibody [HA.C5] (ab18181)
Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).
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Immunocytochemistry/ Immunofluorescence - HA tag antibody [HA.C5] (ab18181)
Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).
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Western blot - HA tag antibody [HA.C5] (ab18181)
All lanes : HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution
Lane 1 : WCE from cell line transfected for HA-tagged protein
Lane 2 : WCE from a cell line transfected with empty vector
Lysates/proteins at 50 µg per lane.
Secondary
HRP conjugated Goat anti-mouse IgG (H+L)
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature. This image is courtesy of an anonymous Abreview
See Abreview
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References for HA tag antibody [HA.C5] (ab18181)
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This product has been used in: (two most recent references)
Denis H et al. Functional connection between deimination and deacetylation of histones. Mol Cell Biol 29:4982-93 (2009). WB, ChIP/Chip. PubMed: 19581286
Igawa T et al. Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings. J Exp Bot 60:3067-73 (2009). WB. PubMed: 19429840
See all 3 publication references for this product.
If you publish research using ab18181 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to HA tag
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HA tag antibody [HA.C5] - more information
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Customer reviews (feedback) regarding HA tag antibody [HA.C5] |
Customer FAQs regarding HA tag antibody [HA.C5] |
Protocols for HA tag antibody [HA.C5] |
Price and availability of products related to HA tag antibody [HA.C5] |
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HA tag antibody [HA.C5] - related products:
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HA tag antibody [HA.C5] - other tools:
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Contact Abcam with a Technical Enquiry about HA tag antibody [HA.C5] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - HA tag antibody [HA.C5] (ab18181)](/index.html?datasheet=18181#ab18181_1.jpg)
![Immunocytochemistry/ Immunofluorescence - HA tag antibody [HA.C5] (ab18181)](/index.html?datasheet=18181#ab18181_2.jpg)
![Western blot - HA tag antibody [HA.C5] (ab18181)](/index.html?datasheet=18181#ab18181_3.jpg)
