You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-HA tag antibody [HA.C5] (ab18181)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-HA tag antibody [HA.C5]
See all HA tag products (36) ...

Description

Mouse monoclonal [HA.C5] to HA tag

Specificity

Recognizes HA-tagged proteins overexpressed in cells, including both amino- and carboxy-termini of targeted proteins in transfected mammalian cells.

Tested applications

ChIP/Chip, WB, ICC, IP, ICC/IFmore details

Immunogen

Synthetic peptide from influenza hemagglutinin epitope: YPYDVPDYA conjugated to KLH.

YPYDVPDYA

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

HA.C5

Isotype

IgG3

Research areas

Tags & Cell Markers >> Epitope Tags >> HA Tag

  • Western blot - HA tag antibody [HA.C5] (ab18181)Western blot - HA tag antibody [HA.C5] (ab18181) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - HA tag antibody [HA.C5] (ab18181)Immunocytochemistry/ Immunofluorescence - HA tag antibody [HA.C5] (ab18181) image (enlarge)

  • Western blot - HA tag antibody [HA.C5] (ab18181)Western blot - HA tag antibody [HA.C5] (ab18181) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab18181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Cellular localization

Not Applicable

Alternative names

  • HA epitope tag antibody
  • hemagglutinin antibody

Anti-HA tag antibody [HA.C5] images:

  Western blot - HA tag antibody [HA.C5] (ab18181)

Western blot - HA tag antibody [HA.C5] (ab18181)

Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).

  Immunocytochemistry/ Immunofluorescence - HA tag antibody [HA.C5] (ab18181)

Immunocytochemistry/ Immunofluorescence - HA tag antibody [HA.C5] (ab18181)

Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).

  Western blot - HA tag antibody [HA.C5] (ab18181)

Western blot - HA tag antibody [HA.C5] (ab18181)

All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution

Lane 1 : WCE from cell line transfected for HA-tagged protein
Lane 2 : WCE from a cell line transfected with empty vector

Lysates/proteins at 50 µg per lane.

Secondary
HRP conjugated Goat anti-mouse IgG (H+L)
developed using the ECL technique

Performed under reducing conditions.

Exposure time : 10 seconds

Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-HA tag antibody [HA.C5] (ab18181)

This product has been referenced in:

  • Frohnert Cet al. SPAG4L/SPAG4L-2 are testis-specific SUN domain proteins restricted to the apical nuclear envelope of round spermatids facing the acrosome. Mol Hum Reprod 17:207-18 (2011).Read more (PubMed: 21159740) »
  • Mankouri Jet al. Optineurin negatively regulates the induction of IFNbeta in response to RNA virus infection. PLoS Pathog 6:e1000778 (2010).Read more (PubMed: 20174559) »

See all 6 publications for this product

Publishing research using ab18181? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"