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Products:Tags & Cell Markers >> Epitope Tags >> HA Tag
Overview
Product name
Anti-HA tag antibody
See all HA tag products (36) ...
Description
Rabbit polyclonal to HA tag
Tested applications
Immunogen
Synthetic peptide HA tag.
.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
-
Western blot
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: 1/500. Can be blocked with HA tag peptide.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Cellular localization
Not Applicable
Alternative names
- HA epitope tag antibody
- hemagglutinin antibody
Anti-HA tag antibody images:
Western blot
All lanes : Anti-HA tag antibody (ab13834) at 1/500 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - expressing HA tag protein (ab20896)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - expressing HA tag protein (ab20896) with HA tag peptide (ab13835) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
References for Anti-HA tag antibody (ab13834)
This product has been referenced in:
- Huang Yet al. UXT-V1 protects cells against TNF-induced apoptosis through modulating complex II formation. Mol Biol Cell 22:1389-97 (2011). WB; Human.Read more (PubMed: 21307340) »
- Alhamidi Met al. Fukutin-Related Protein Resides in the Golgi Cisternae of Skeletal Muscle Fibres and Forms Disulfide-Linked Homodimers via an N-Terminal Interaction. PLoS One 6:e22968 (2011). WB.Read more (PubMed: 21886772) »
See all 3 publications for this product
Publishing research using ab13834? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"