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ab13835 |
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ab13835 |
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Read our guarantee »Products:Tags & Cell Markers >> Epitope Tags >> HA Tag
Anti-HA tag antibody
See all HA tag products (36) ...
Rabbit polyclonal to HA tag
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Western blot
(enlarge)
Our Abpromise guarantee covers the use of ab13834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500. Can be blocked with HA tag peptide.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Not Applicable
Western blot

All lanes : Anti-HA tag antibody (ab13834) at 1/500 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - expressing HA tag protein (ab20896)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - expressing HA tag protein (ab20896) with HA tag peptide (ab13835) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
This product has been referenced in:
See all 3 publications for this product
Publishing research using ab13834? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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All lanes : Anti-HA tag antibody (ab13834) at 1/500 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - expressing HA tag protein (ab20896)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - expressing HA tag protein (ab20896) with HA tag peptide (ab13835) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
2
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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