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Product Name 

HA tag antibody

 

See all HA tag antibodies (27)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to HA tag

Immunogen 

Synthetic peptide HA tag.

.

Reacts with 
(species key)

N/A

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: 1/500. Can be blocked with HA tag peptide.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Tags & Cell Markers >> Epitope Tags >> HA Tag

Relevance 

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

 

Alternative names HA tag antibody (ab13834)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.50 - 0.70 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HA tag antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for HA tag antibody images, references, products related to ab13834 and other tools.

 

HA tag antibody images:

Product image  Western blot

Western blot

All lanes : HA tag antibody (ab13834) at 1/500 dilution

Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - expressing HA tag protein (ab20896)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - expressing HA tag protein (ab20896) with HA tag peptide (ab13835) at 1 µg/ml

Lysates/proteins at 10 µg per lane.

Secondary
Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution

Performed under reducing conditions.

Observed band size : 55 kDa (why is the actual band size different from the predicted?)

 

References for HA tag antibody (ab13834)

Specific publication references   This product has been used in: (two most recent references)

Rismanchi N et al. STAM adaptor proteins interact with COPII complexes and function in ER-to-Golgi trafficking. Traffic 10:201-17 (2009). WB. PubMed: 19054391

Ichimura K et al. IDH1 mutations are present in the majority of common adult gliomas but rare in primary glioblastomas. Neuro Oncol 11:341-7 (2009). WB. PubMed: 19435942
If you publish research using ab13834 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to HA tag

 

HA tag antibody - more information

Customer reviews (feedback) regarding HA tag antibody

Customer FAQs regarding HA tag antibody

Protocols for HA tag antibody

Price and availability of products related to HA tag antibody

 

HA tag antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Immunizing Peptide (Blocking) for HA tag antibody

ab13835

  

HA tag peptide

 

Immunizing Peptide for HA tag antibody

 

Positive Controls for HA tag antibody

ab20896

  

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - expressing HA tag protein

 

Compatible Secondaries for HA tag antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other HA tag Antibodies

See all HA tag antibodies (27)...

 

HA tag antibody - other tools:

Contact Abcam with a Technical Enquiry about HA tag antibody

 

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