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Overview

  • Product nameHA1 (H9N2) protein
  • Protein descriptionRecombinant Full-Length HA1 (H9N2 A/Hong-Kong/1073/99) is glycosylated with N-linked sugars, produced using baculovirus vectors in insect cells. Swiss Prot Id: Q9ICY5, NCBI AJ404626.
  • Expression hostBaculovirus infected insect cells

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Purification notessterile filtered.
  • FormLiquid
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.01% Tween 20, 150mM Sodium chloride, 10mM Sodium phosphate, pH 7.0
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab67740 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
    ELISA
  • Application notesELISA: Use at a concentration of 1 µg/well.
    WB: Use at a concentration of 0.1 - 1µg per strip. Runs as a band of approximately 72 kDa (predicted molecular weight: 63 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Hemagglutinin
  • RelevanceHemagglutinin (HA) is a class I viral fusion protein from the Influenza virus. It is a major glycoprotein, comprising over 80% of the envelope proteins present in the virus particle. HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell, and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. The HA protein is a homotrimer of disulfide-linked HA1-HA2. It also plays a major role in the determination of host range restriction and virulence. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. Influenza A subtypes are classified based on the combination of the virus coat glycoproteins, hemagglutinin (HA) and neuraminidase (NA). H9N2 influenza A viruses circulate worldwide. Since the mid-1990s, H9 viruses have become adapted to land-based birds and have crossed sporadically to pigs and humans, causing mild respiratory disease. Importantly, some of the currently circulating H9N2 viruses bind to sialic acid receptors linked to galactose in the {alpha}-2,6 conformation, which is the preferential binding pattern of human influenza viruses. Thus, these H9N2 viruses possess one of the key elements needed to establish stable lineages in humans.
  • Cellular localizationviral envelope; apical plasma membrane. Associated with glycosphingolipid- and cholesterol-enriched detergent-resistant lipid rafts (By similarity).

    • References for HA1 (H9N2) protein (ab67740)

    ab67740 has not yet been referenced specifically in any publications.

    Publishing research using ab67740 ? Please let us know so that we can cite the reference in this datasheet

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    Is the recombinant protein raised in Sf9 cells? Plus are the insect cells propagated in FBS or BSA (or any other animal sources) serum? If so, I'll need to get a permit to order this.

    Thank you for your inquiry. The protein is made in SF+ cells - a proprietary cell line. Also, the cell media is serum free. I hope this information helps. Please contact us with any other questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"