Anti-HABP2 antibody (ab232778)
Key features and details
- Rabbit polyclonal to HABP2
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-HABP2 antibody
See all HABP2 primary antibodies -
Description
Rabbit polyclonal to HABP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human HABP2 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: Q14520 -
Positive control
- IHC-P: Human rectum tissue. WB: Recombinant human HABP2 protein; Pig liver, kidney and small intestine lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab232778 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 63 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 63 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Cleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form. Activates coagulation factor VII. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 3 EGF-like domains.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsProteolytic cleavage at Gly-23 or Met-27 can give rise to the 50 kDa heavy chain and cleavage at Arg-313 or Lys-319 can give rise to the 27 kDa light chain. The heavy chain can undergo further proteolytic cleavage at Lys-169 or Arg-170 to give rise to 2 inactive 26 kDa fragments and the light chain can undergo further proteolytic cleavage at Arg-480 to give rise to inactive 17 kDa and 8 kDa fragments. -
Cellular localization
Secreted. Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form. - Information by UniProt
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Database links
- Entrez Gene: 3026 Human
- Omim: 603924 Human
- SwissProt: Q14520 Human
- Unigene: 422542 Human
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Alternative names
- Factor seven activating protease antibody
- Factor seven-activating protease antibody
- Factor VII activating protein antibody
see all
Images
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Formalin-fixed, paraffin-embedded human rectum tissue stained for HABP2 using ab232778 at 20µg/mL in immunohistochemical analysis. DAB staining.
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Anti-HABP2 antibody (ab232778) at 2 µg/ml + Recombinant human HABP2 protein.
Predicted band size: 63 kDa -
All lanes : Anti-HABP2 antibody (ab232778) at 1 µg/ml
Lane 1 : Pig kidney tissue lysate
Lane 2 : Pig intestine tissue lysate
Lane 3 : Pig liver tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/1000 dilution
Predicted band size: 63 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab232778 has not yet been referenced specifically in any publications.