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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-HABP2 antibody
See all HABP2 products (2) ...
Description
Rabbit polyclonal to HABP2
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 288-337 (PTEPSTKLPG FDSCGKTEIA ERKIKRIYGG FKSTAGKHPW QASLQSSLPL) of human HABP2 (NP_004123).
PTEPSTKLPG FDSCGKTEIA ERKIKRIYGG FKSTAGKHPW QASLQSSLPL
Positive control
MCF7 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
-
Western blot - HABP2 antibody (ab81490)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay, 1:62500
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Cleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form. Activates coagulation factor VII.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the peptidase S1 family.
Contains 3 EGF-like domains.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain.
Post-translational
modifications
Proteolytic cleavage at Gly-23 or Met-27 can give rise to the 50 kDa heavy chain and cleavage at Arg-313 or Lys-319 can give rise to the 27 kDa light chain. The heavy chain can undergo further proteolytic cleavage at Lys-169 or Arg-170 to give rise to 2 inactive 26 kDa fragments and the light chain can undergo further proteolytic cleavage at Arg-480 to give rise to inactive 17 kDa and 8 kDa fragments.
Cellular localization
Secreted. Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.
Target information above from: UniProt accessionQ14520
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Factor seven activating protease antibody
- Factor seven-activating protease antibody
- Factor VII activating protein antibody
see all
Database links
- Entrez Gene: 507993 Cow
- Entrez Gene: 3026 Human
- Entrez Gene: 226243 Mouse
- Entrez Gene: 292126 Rat
- Omim: 603924 Human
- SwissProt: Q5E9Z2 Cow
- SwissProt: Q14520 Human
- SwissProt: Q8K0D2 Mouse
see all
Anti-HABP2 antibody images:
Western blot - HABP2 antibody (ab81490)
Anti-HABP2 antibody (ab81490) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 63 kDa
Observed band size : 63 kDa
Additional bands at : 27 kDa,28 kDa. We are unsure as to the identity of these extra bands.
References for Anti-HABP2 antibody (ab81490)
ab81490 has not yet been referenced specifically in any publications.
Publishing research using ab81490? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"