Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase
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Anti-HACE1 antibody (ab83776) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 102 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
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