Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase
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We are working on Hace1 a have not found a good antibody. I would like to test your antibody using lysets from Hace1 +/+ and -/- MEFs and if it is good we will place an order.
Thank you, |
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ANSWER: |
Thank you for contacting us and for your interest in our products.
I am sorry to confirm that as far as we are aware, ab83776 Anti-HACE1 antibody has never been tested with samples from mouse. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention.
The mouse HACE1 protein sequence does have high alignment to the immunogen used for this antibody. Therefore, it is likely (predicted) to react, but we are not able to guarantee this without further testing.
If you would like to test the antibody in this untested species, please contact me by replying to this message prior to the purchase as you may be eligible for our testing discount program.
Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.
To find out more about our Abreview system, please see the following link:
http://www.abcam.com/abreviews
I hope this information is helpful. Please do not hesitate to contact me for any further advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-HACE1 antibody (ab83776) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 102 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
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