Synthetic peptide corresponding to a region within the internal amino acids 792-841 (DVSDWIKNT EYTSGYERED PVIQWFWEVV EDITQEERVL LLQFVTGSSR of Human HACE1 (NP_065822).
BLAST 'DVSDWIKNTE YTSGYEREDP VIQWFWEVVE DITQEERVLL LQFVTGSSRV ' with or
Recommended dilutions ELISA: Use at an assay dependent dilution. Titre in peptide based assay is 1/1562500. WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 102 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
E3 ubiquitin-protein ligase that may function in cellular proteins degradation. HACE1 has also been shown to be down-regulated in many human tumours and is a putative candidate tumor suppressor.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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See below for HACE1 antibody images, references, products related to ab83776 and other tools.
HACE1 antibody images:
Western blot - HACE1 antibody (ab83776)
HACE1 antibody (ab83776) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Pricing information loading
Western blot - HACE1 antibody (ab83776)