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Western blot - TFPI antibody (ab54477)
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Immunocytochemistry/ Immunofluorescence-HADHA antibody(ab54477)
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Product Name
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HADHA antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HADHA
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Immunogen
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Synthetic peptide surrounding amino acid 750 (Human)
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell lysate
3T3 cell lysate
Rat kidney lysate
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Cellular localization
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Mitochondrial
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Relevance
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Mitochondrial Trifunctional Protein (TFP) is a multienzyme complex of the beta-oxidation cycle. TFP deficiency is a clinically heterogeneous disorder with phenotypes of different severity. The spectrum of diseases range from severe neonatal/infantile cardiomyopathy and early death to
mild chronic progressive sensorimotor poly-neuropathy with episodic rhabdomyolysis. Human TFP is an octomer composed of four alpha-subunits and four beta-subunits. Mutations in either subunits may result in general TFP deficiency with reduced activity of all enzymes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.5% BSA, PBS Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Thimerosal MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HADHA antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HADHA antibody images, references, products related to ab54477 and other tools.
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HADHA antibody images:
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 Western blot - TFPI antibody (ab54477)
All lanes : HADHA antibody (ab54477) at 4 µg/ml
Lane 1 : Jurkat cell lysate 30-50 ug/lane.
Lane 2 : Jurkat cell lysate 30-50 ug/lane.
Lane 3 : 3T3 cell lysate 30-50 ug/lane.
Lane 4 : Rat kidney lysate 30-50 ug/lane.
Secondary
Anti-Rabbit IgG, HRP-Linked Antibody at 1/5000 dilution
Predicted band size : 83 kDa
Observed band size : 83 kDa
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Immunocytochemistry/ Immunofluorescence-HADHA antibody(ab54477)
ICC/IF image of ab54477 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54477, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to HADHA
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HADHA antibody - more information
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Customer reviews (feedback) regarding HADHA antibody |
Customer FAQs regarding HADHA antibody |
Protocols for HADHA antibody |
Price and availability of products related to HADHA antibody |
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HADHA antibody - related products:
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HADHA antibody - other tools:
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Contact Abcam with a Technical Enquiry about HADHA antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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