![Other - Anti-HADHB antibody [5C3AB7 ] (ab110301) image](#HADHB-Primary-antibodies-ab110301-1.gif)
![Immunocytochemistry - HADHB antibody [5C3AB7 ] (ab110301) image](#HADHB-Primary-antibodies-ab110301-5.jpg)
![Flow Cytometry - HADHB antibody [5C3AB7 ] (ab110301) image](#HADHB-Primary-antibodies-ab110301-4.gif)
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Product name
Anti-HADHB antibody [5C3AB7 ]
See all HADHB products (5) ...
Description
Mouse monoclonal [5C3AB7 ] to HADHB
Tested applications
In-Cell ELISA, IP, WB, Flow Cyt, ICCmore details
Cross reactivity
Reacts with
Human
Immunogen
The immunogen is sucrose gradient fraction 1+2 from Human liver mitochondria.
Positive control
HepG2 and HLM cell lysates; HDFn and HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab110301 was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification.
Clonality
Monoclonal
Clone number
5C3AB7
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipid metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
In-Cell ELISA: Use a concentration of 1 µg/ml.
IP: Use at an assay dependent dilution.
WB: Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa.
Flow Cyt: Use a concentration of 1 µg/ml.
ICC: Use a concentration of 1 µg/ml.
Target
Pathway
Lipid metabolism; fatty acid beta-oxidation.
Involvement in disease
Defects in HADHB are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015]. The clinical manifestations are very variable and include hypoglycemia, cardiomyopathy and sudden death. Phenotypes with mainly hepatic and neuromyopathic involvement can also be distinguished. Biochemically, TFP deficiency is defined by the loss of all three enzyme activities of the TFP complex.
Sequence similarities
Belongs to the thiolase family.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionP55084
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2 enoyl Coenzyme A (CoA) hydratase beta subunit antibody
- 3 ketoacyl Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein beta subunit antibody
- 3 ketoacyl Coenzyme A thiolase antibody
see all
Database links
- Entrez Gene: 3032 Human
- Omim: 143450 Human
- SwissProt: P55084 Human
- Unigene: 515848 Human
Anti-HADHB antibody [5C3AB7 ] images:
Other - Anti-HADHB antibody [5C3AB7 ] (ab110301)
![Other - Anti-HADHB antibody [5C3AB7 ] (ab110301)](/ps/datasheet/images/110/ab110301/HADHB-Primary-antibodies-ab110301-1.gif)
As a Western blot antibody, MS733 works with HepG2 (human) cells.
Immunocytochemistry - HADHB antibody [5C3AB7 ] (ab110301)
![Immunocytochemistry - HADHB antibody [5C3AB7 ] (ab110301)](/ps/datasheet/images/110/ab110301/HADHB-Primary-antibodies-ab110301-5.jpg)
Immunocytochemistry analysis using ab110301 at 1µg/ml staining HADHB in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized).
The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue).
Flow Cytometry - HADHB antibody [5C3AB7 ] (ab110301)
![Flow Cytometry - HADHB antibody [5C3AB7 ] (ab110301)](/ps/datasheet/images/110/ab110301/HADHB-Primary-antibodies-ab110301-4.gif)
Flow cytometric analysis using ab110301 at 1µg/ml staining HADHB in HeLa cells (blue). Isotype control antibody (red).
Immunoprecipitation - Anti-HADHB antibody [5C3AB7 ] (ab110301)
![Immunoprecipitation - Anti-HADHB antibody [5C3AB7 ] (ab110301)](/ps/datasheet/images/110/ab110301/HADHB-Primary-antibodies-ab110301-6.gif)
As an IP antibody, MS733 works with human samples.
References for Anti-HADHB antibody [5C3AB7 ] (ab110301)
ab110301 has not yet been referenced specifically in any publications.
Publishing research using ab110301? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"