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Anti-HAGH antibody (ab55230) at 5 µg/ml + 0.2 µg/lane of partial recombinant HAGH protein with a tag
Secondary
Goat anti mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Predicted band size : 29 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
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