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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab55230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a dilutions 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 29 kDa).

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceHydroxyacylglutathione hydrolase (HAGH) is a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate.
    • Cellular localizationCytoplasmic and Mitochondrial
    • Database links
      • Alternative names
          GLO2 antibodyGLX II antibodyGlyoxalase II antibody
          HAGH antibodyHAGH1 antibodyHydroxyacyl glutathione hydrolase antibodyHydroxyacylglutathione hydrolase, mitochondrial antibody
        see all

      Anti-HAGH antibody images

      • Western blot - HAGH antibody (ab55230)
        Western blot - HAGH antibody (ab55230)
        Anti-HAGH antibody (ab55230) at 5 µg/ml + 0.2 µg/lane of partial recombinant HAGH protein with a tag

        Secondary
        Goat anti mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

        Predicted band size : 29 kDa
        Observed band size : 40 kDa (why is the actual band size different from the predicted?)

      References for Anti-HAGH antibody (ab55230)

      ab55230 has not yet been referenced specifically in any publications.

      Publishing research using ab55230 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"