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ab47013 |
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Read our guarantee »Anti-HAND1 antibody
See all HAND1 products (5) ...
Rabbit polyclonal to HAND1
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Pig
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human HAND1.
(Peptide available as ab47013.)
This antibody gave a positive signal in the following Human tissue lysates: Brain, Heart.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Western blot - HAND1 antibody (ab46822)
(enlarge)
Our Abpromise guarantee covers the use of ab46822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 25.8 kDa (predicted molecular weight: 25.8 kDa).
Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box).
Heart.
Contains 1 basic helix-loop-helix (bHLH) domain.
Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity.
Nucleus > nucleoplasm. Nucleus > nucleolus. Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity.
Target information above from: UniProt accessionO96004
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - HAND1 antibody (ab46822)

All lanes : Anti-HAND1 antibody (ab46822) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 25.8 kDa
Observed band size : 25.8 kDa
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
ab46822 has not yet been referenced specifically in any publications.
Publishing research using ab46822? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:

All lanes : Anti-HAND1 antibody (ab46822) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 25.8 kDa
Observed band size : 25.8 kDa
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
1
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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