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Overview
Product name
Anti-HAND1 antibody
See all HAND1 products (5) ...
Description
Rabbit polyclonal to HAND1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human HAND1.
(Peptide available as ab47013.)
Positive control
This antibody gave a positive signal in the following Human tissue lysates: Brain, Heart.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - HAND1 antibody (ab46822)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab46822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 25.8 kDa (predicted molecular weight: 25.8 kDa).
Target
Function
Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box).
Tissue specificity
Heart.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity.
Cellular localization
Nucleus > nucleoplasm. Nucleus > nucleolus. Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity.
Target information above from: UniProt accessionO96004
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- autonomic nervous system and neural crest derivatives-expressed protein 1 antibody
- heart antibody
- Basic helix loop helix transcription factor HAND1 antibody
see all
Database links
- Entrez Gene: 9421 Human
- Entrez Gene: 15110 Mouse
- Entrez Gene: 59112 Rat
- Omim: 602406 Human
- SwissProt: Q90691 Chicken
- SwissProt: O96004 Human
- SwissProt: Q64279 Mouse
- SwissProt: P57100 Rabbit
see all
Anti-HAND1 antibody images:
Western blot - HAND1 antibody (ab46822)
All lanes : Anti-HAND1 antibody (ab46822) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 25.8 kDa
Observed band size : 25.8 kDa
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
References for Anti-HAND1 antibody (ab46822)
ab46822 has not yet been referenced specifically in any publications.
Publishing research using ab46822? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"