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Western blot - HAND1 antibody (ab46822)
Western blot - HAND1 antibody (ab46822)
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Product Name 

HAND1 antibody

 

See all HAND1 antibodies (3)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to HAND1

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human HAND1.

(Peptide available as ab47013.)

Reacts with 

Hu

Predicted to react 
(species key)

Cow, Pig, Rb, Shp
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 25.8 kDa (predicted molecular weight: 25.8 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in the following Human tissue lysates: Brain, Heart.

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Lineage specification >> Ectoderm
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Stem Cells >> Lineage Markers >> Ectoderm
Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

HAND1 is a transcription regulator and plays an essential role in early trophoblast differentiation and in cardiac morphogenesis. In the adult, it may be required for ongoing expression of cardiac-specific genes.

 

Alternative names HAND1 antibody (ab46822)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

9421    (Human)
15110    (Mouse)
59112    (Rat)

Omim

    

602406    (Human)

SwissProt

    

Q90691    (Chicken)
O96004    (Human)
Q64279    (Mouse)
P57100    (Rabbit)
P97832    (Rat)
Q28555    (Sheep)

Unigene

    

152531    (Human)
39340    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HAND1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for HAND1 antibody images, references, products related to ab46822 and other tools.

 

HAND1 antibody images:

Product image  Western blot - HAND1 antibody (ab46822)

Western blot - HAND1 antibody (ab46822)

All lanes : HAND1 antibody (ab46822) at 1 µg/ml

Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 25.8 kDa
Observed band size : 25.8 kDa
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

 
 

Search PubMed (MEDLINE) for references to HAND1

 

HAND1 antibody - more information

Customer reviews (feedback) regarding HAND1 antibody

Customer FAQs regarding HAND1 antibody

Protocols for HAND1 antibody

Price and availability of products related to HAND1 antibody

 

HAND1 antibody - related products:

Products for Western blot

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Lysates

 

Immunizing Peptide for HAND1 antibody

ab47013

  

HAND1 peptide

 

Compatible Secondaries for HAND1 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other HAND1 Antibodies

See all HAND1 antibodies (3)...

 

HAND1 antibody - other tools:

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