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Overview
Product name
Anti-HAND1 antibody
See all HAND1 products (5) ...
Description
Rabbit polyclonal to HAND1
Tested applications
Cross reactivity
Predicted to work with
Mouse, Human
Immunogen
3 synthetic peptides (Human) conjugated to KLH
Positive control
Induced culture of E.coli. Heart tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Whole serum
Purity
Whole antiserum
Purification notes
Crude serum, final bleed.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - HAND1 antibody (ab86009)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Predicted molecular weight: 24 kDa.
Target
Function
Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box).
Tissue specificity
Heart.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity.
Cellular localization
Nucleus > nucleoplasm. Nucleus > nucleolus. Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity.
Target information above from: UniProt accessionO96004
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- autonomic nervous system and neural crest derivatives-expressed protein 1 antibody
- heart antibody
- Basic helix loop helix transcription factor HAND1 antibody
see all
Database links
- Entrez Gene: 9421 Human
- Entrez Gene: 15110 Mouse
- Omim: 602406 Human
- SwissProt: O96004 Human
- SwissProt: Q64279 Mouse
- Unigene: 152531 Human
- Unigene: 4746 Mouse
Anti-HAND1 antibody images:
Western blot - HAND1 antibody (ab86009)
All lanes : Anti-HAND1 antibody (ab86009) at 1/1000 dilution
Lane 1 : Uninduced culture
Lane 2 : Induced culture of E.coli
Secondary
anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size : 24 kDa
Plasmid name： pBAD-DEST49.
References for Anti-HAND1 antibody (ab86009)
ab86009 has not yet been referenced specifically in any publications.
Publishing research using ab86009? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"