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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-HAND2 antibody - Carboxyterminal end
See all HAND2 products (3) ...
Description
Rabbit polyclonal to HAND2 - Carboxyterminal end
Tested applications
ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (10-30 aa in length) at the C-terminal of last 50 aa of human HAND2 conjugated to KLH
Positive control
Human hepatocarcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab60037 is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Neuroscience >> Neurology process >> Neurogenesis
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HAND2 antibody (ab60037)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab60037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/50 - 1/100.
Target
Function
Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3'.
Tissue specificity
Heart.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Developmental stage
Expressed in the fetal heart.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP61296
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- autonomic nervous system and neural crest derivatives-expressed protein 2 antibody
- heart antibody
- AI225906 antibody
see all
Database links
- Entrez Gene: 9464 Human
- Omim: 602407 Human
- SwissProt: P61296 Human
- Unigene: 388245 Human
Anti-HAND2 antibody - Carboxyterminal end images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HAND2 antibody (ab60037)
ab60037 at 1/50 dilution staining HAND2 in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. A peroxidase-conjugated secondary antibody was used, followed by AEC staining.
References for Anti-HAND2 antibody - Carboxyterminal end (ab60037)
ab60037 has not yet been referenced specifically in any publications.
Publishing research using ab60037? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"