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Read our guarantee »Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Anti-HAO1 antibody
See all HAO1 products (3) ...
Mouse polyclonal to HAO1
Reacts with
Human
Full length human HAO1 protein (NP_060015.1)
HAO1 transfected 293T cell lysate
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein G purified
Polyclonal
IgG
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Western blot - HAO1 antibody (ab69298)
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Our Abpromise guarantee covers the use of ab69298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
Liver.
Organic acid metabolism; glycolate degradation; 3-phospho-D-glycerate from glycolate: step 1/4.
Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.
Contains 1 FMN hydroxy acid dehydrogenase domain.
Peroxisome.
Target information above from: UniProt accessionQ9UJM8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - HAO1 antibody (ab69298)

All lanes : Anti-HAO1 antibody (ab69298) at 1/500 dilution
Lane 1 : HAO1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 41 kDa
Observed band size : 41 kDa
ab69298 has not yet been referenced specifically in any publications.
Publishing research using ab69298? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
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All lanes : Anti-HAO1 antibody (ab69298) at 1/500 dilution
Lane 1 : HAO1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 41 kDa
Observed band size : 41 kDa
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