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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Other ECM Proteins
Overview
Product name
Anti-HAPLN4 antibody
See all HAPLN4 products (3) ...
Description
Mouse polyclonal to HAPLN4
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Chimpanzee, Rhesus monkey
Immunogen
Recombinant fragment with tag: QRGRKKVVHV LEGESGSVVV QTAPGQVVSH RGGTIVLPCR YHYEAAAHGH DGVRLKWTKV VDPLAFTDVF VALGPQHRAF GSYRGRAELQ GDGPGDASLV LRNVT, corresponding to amino acids 30-135 of Human HAPLN4
QRGRKKVVHV LEGESGSVVV QTAPGQVVSH RGGTIVLPCR YHYEAAAHGH DGVRLKWTKV VDPLAFTDVF VALGPQHRAF GSYRGRAELQ GDGPGDASLV LRNVT
Positive control
Partial recombinant HAPLN4.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Other ECM Proteins
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Western blot - HAPLN4 antibody (ab66149)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
WB: 1/1000 - 1/2500. Detects a band of approximately 38 kDa (predicted molecular weight: 43 kDa).
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Binds to hyaluronic acid and may be involved in formation of the extracellular matrix.
Tissue specificity
Expressed predominantly in brain.
Sequence similarities
Belongs to the HAPLN family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 2 Link domains.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionQ86UW8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Brain link protein 2 antibody
- BRAL 2 antibody
- BRAL2 antibody
see all
Database links
- Entrez Gene: 404037 Human
- Entrez Gene: 330790 Mouse
- Entrez Gene: 361129 Rat
- SwissProt: Q86UW8 Human
- SwissProt: Q80WM4 Mouse
- Unigene: 367829 Human
- Unigene: 152048 Mouse
Anti-HAPLN4 antibody images:
Western blot - HAPLN4 antibody (ab66149)
Predicted band size : 43 kDa
Western blot against tagged recombinant protein immunogen using ab66149 HAPLN4 antibody at 1/1000 dilution. Predicted band size of immunogen is 38 kDa.
References for Anti-HAPLN4 antibody (ab66149)
ab66149 has not yet been referenced specifically in any publications.
Publishing research using ab66149? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"