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Products:Immunology >> Immune System Diseases >> Autoimmune
Overview
Product name
Anti-HARS antibody - Aminoterminal end
See all HARS products (5) ...
Description
Rabbit polyclonal to HARS - Aminoterminal end
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Positive control
K562 cell line lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Immunology >> Immune System Diseases >> Autoimmune
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/50 - 1/100.Predicted molecular weight: 56 kDa.
IHC-P: 1/10 - 1/50.
Target
Relevance
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
Cellular localization
Cytoplasmic
Alternative names
- EC 6.1.1.21 antibody
- FLJ20491 antibody
- HisRS antibody
see all
Database links
- Entrez Gene: 3035 Human
- Omim: 142810 Human
- SwissProt: P12081 Human
- Unigene: 528050 Human
Anti-HARS antibody - Aminoterminal end images:
Western blot - HARS antibody (ab71305)
Anti-HARS antibody - Aminoterminal end (ab71305) at 1/60 dilution + K562 cell lysate at 35 µg
Predicted band size : 56 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-HARS antibody - Aminoterminal end(ab71305)
ab71305 at 1/50 dilution staining HARS - Aminoterminal in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
References for Anti-HARS antibody - Aminoterminal end (ab71305)
ab71305 has not yet been referenced specifically in any publications.
Publishing research using ab71305? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"