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Anti-HARS antibody [HARSA6] (ab50835)

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Overview

Product name

Anti-HARS antibody [HARSA6]
See all HARS products (5) ...

Description

Mouse monoclonal [HARSA6] to HARS

Specificity

This antibody reacts with HARS

Tested applications

IHC-P, ICC, IP, Flow Cyt, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

HARS recombinant fragment (Human), containing 50-200 AAs from near the C-terminal.

Positive control

F2408 whole cell lysate, HeLa cells and HeLa whole cell lysate

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

Purified through Protein G, from culture supenatant of hybridoma cultured in a meduim containing bovine IgG-depleted (~95%) fetal bovine serum. Filtered through a 0.22µm membrane.

Clonality

Monoclonal

Clone number

HARSA6

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Immunology >> Immune System Diseases >> Autoimmune

  • Western blot - HARS antibody [HARSA6] (ab50835)Western blot - HARS antibody [HARSA6] (ab50835) image (enlarge)

  • Immunocytochemistry - HARS antibody [HARSA6] (ab50835)Immunocytochemistry - HARS antibody [HARSA6] (ab50835) image (enlarge)

  • Flow Cytometry - HARS antibody [HARSA6] (ab50835)Flow Cytometry - HARS antibody [HARSA6] (ab50835) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • 1 Image

    ICC

     ICC: 1/10

    ICC: 1/10

  • IP

     IP: 1/100

    IP: 1/100

  • 1 Image

    Flow Cyt

     Flow Cyt: 1/10

    Flow Cyt: 1/10

  • ShowHide2 Images

    WB

     WB: 1/100Detects a band ...Read more →

    WB: 1/100Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).

Target

Relevance

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Cellular localization

Cytoplasmic

Alternative names

  • EC 6.1.1.21 antibody
  • FLJ20491 antibody
  • HisRS antibody
  • histidine translase antibody
  • Histidine tRNA ligase antibody
  • Histidyl tRNA synthetase antibody
  • HRS antibody
  • Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds antibody
see all

Database links

Anti-HARS antibody [HARSA6] images:

  Western blot - HARS antibody [HARSA6] (ab50835)

Western blot - HARS antibody [HARSA6] (ab50835)

Anti-HARS antibody [HARSA6] (ab50835) at 1/100 dilution + Hela whole cell lysate at 25 µg

Secondary
Mouse IgG antibody at 1/2500 dilution

Predicted band size : 46 kDa
Observed band size : 50,100 kDa (why is the actual band size different from the predicted?)

  Immunocytochemistry - HARS antibody [HARSA6] (ab50835)

Immunocytochemistry - HARS antibody [HARSA6] (ab50835)

ab50835 at a 1/10 dilution staining HARS in Hela cells, using Alexa Fluor®488 Goat Anti-mouse IgG at 1/200 dilution.

  Flow Cytometry - HARS antibody [HARSA6] (ab50835)

Flow Cytometry - HARS antibody [HARSA6] (ab50835)

ab50835 at a 1/10 dilution staining HARS using Alexa Fluor® 488 Goat Anti-mouse IgG at 1/400 dilution.

  Western blot - HARS antibody [HARSA6] (ab50835)

Western blot - HARS antibody [HARSA6] (ab50835)

Anti-HARS antibody [HARSA6] (ab50835) at 1/100 dilution + F2408 whole cell lysate at 25 µg

Secondary
Mouse IgG antibody at 1/2500 dilution

Predicted band size : 46 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HARS antibody [HARSA6] (ab50835)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HARS antibody [HARSA6] (ab50835)

IHC image of ab50835 staining in human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50835, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-HARS antibody [HARSA6] (ab50835)

ab50835 has not yet been referenced specifically in any publications.

Publishing research using ab50835? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"