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Western blot - HARS antibody [HARSA6] (ab50835)
![Western blot - HARS antibody [HARSA6] (ab50835)](/ps/datasheet/images/ab50835_1.jpg)
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Immunocytochemistry - HARS antibody [HARSA6] (ab50835)
![Immunocytochemistry - HARS antibody [HARSA6] (ab50835)](/ps/datasheet/images/ab50835_2.jpg)
(enlarge)
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Flow Cytometry - HARS antibody [HARSA6] (ab50835)
![Flow Cytometry - HARS antibody [HARSA6] (ab50835)](/ps/datasheet/images/ab50835_4.jpg)
(enlarge)
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Product Name
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HARS antibody [HARSA6]
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See all HARS antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [HARSA6] to HARS
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Immunogen
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HARS recombinant fragment (Human), containing 50-200 AAs from near the C-terminal.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody reacts with HARS
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Tested applications
(see key)
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Flow Cyt, ICC, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/10.
ICC: 1/10.
IP: 1/100.
WB: 1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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F2408 whole cell lysate, HeLa cells and HeLa whole cell lysate
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Immunology >> Immune System Diseases >> Autoimmune
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Relevance
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Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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HARSA6
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified through Protein G, from culture supenatant of hybridoma cultured in a meduim containing bovine IgG-depleted (~95%) fetal bovine serum. Filtered through a 0.22µm membrane.
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HARS antibody [HARSA6] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HARS antibody [HARSA6] images, references, products related to ab50835 and other tools.
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HARS antibody [HARSA6] images:
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 Western blot - HARS antibody [HARSA6] (ab50835)
HARS antibody [HARSA6] (ab50835) at 1/100 dilution + Hela whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size : 46 kDa
Observed band size : 50,100 kDa (why is the actual band size different from the predicted?)
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Immunocytochemistry - HARS antibody [HARSA6] (ab50835)
ab50835 at a 1/10 dilution staining HARS in Hela cells, using Alexa Fluor®488 Goat Anti-mouse IgG at 1/200 dilution.
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Flow Cytometry - HARS antibody [HARSA6] (ab50835)
ab50835 at a 1/10 dilution staining HARS using Alexa Fluor® 488 Goat Anti-mouse IgG at 1/400 dilution.
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Western blot - HARS antibody [HARSA6] (ab50835)
![Western blot - HARS antibody [HARSA6] (ab50835)](/ps/datasheet/images/ab50835_5.jpg)
HARS antibody [HARSA6] (ab50835) at 1/100 dilution + F2408 whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size : 46 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to HARS
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HARS antibody [HARSA6] - more information
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Customer reviews (feedback) regarding HARS antibody [HARSA6] |
Customer FAQs regarding HARS antibody [HARSA6] |
Protocols for HARS antibody [HARSA6] |
Price and availability of products related to HARS antibody [HARSA6] |
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HARS antibody [HARSA6] - related products:
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HARS antibody [HARSA6] - other tools:
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Contact Abcam with a Technical Enquiry about HARS antibody [HARSA6] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - HARS antibody [HARSA6] (ab50835)](/index.html?datasheet=50835#ab50835_1.jpg)
![Immunocytochemistry - HARS antibody [HARSA6] (ab50835)](/index.html?datasheet=50835#ab50835_2.jpg)
![Flow Cytometry - HARS antibody [HARSA6] (ab50835)](/index.html?datasheet=50835#ab50835_4.jpg)
