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Read our guarantee »Products:Immunology >> Immune System Diseases >> Autoimmune
Anti-HARS antibody [HARSA6]
See all HARS products (5) ...
Mouse monoclonal [HARSA6] to HARS
This antibody reacts with HARS
IHC-P, ICC, IP, Flow Cyt, WBmore details
Reacts with
Human
HARS recombinant fragment (Human), containing 50-200 AAs from near the C-terminal.
F2408 whole cell lysate, HeLa cells and HeLa whole cell lysate
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS
Concentration information loading...
Protein G purified
Purified through Protein G, from culture supenatant of hybridoma cultured in a meduim containing bovine IgG-depleted (~95%) fetal bovine serum. Filtered through a 0.22µm membrane.
Monoclonal
HARSA6
IgG1
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Immunology >> Immune System Diseases >> Autoimmune
Our Abpromise guarantee covers the use of ab50835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC: 1/10
IP: 1/100
Flow Cyt: 1/10
WB: 1/100Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
Cytoplasmic
Western blot - HARS antibody [HARSA6] (ab50835)
![Western blot - HARS antibody [HARSA6] (ab50835)](/ps/datasheet/Images/50/ab50835/ab50835_1.jpg)
Anti-HARS antibody [HARSA6] (ab50835) at 1/100 dilution + Hela whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size : 46 kDa
Observed band size : 50,100 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry - HARS antibody [HARSA6] (ab50835)
![Immunocytochemistry - HARS antibody [HARSA6] (ab50835)](/ps/datasheet/Images/50/ab50835/ab50835_2.jpg)
ab50835 at a 1/10 dilution staining HARS in Hela cells, using Alexa Fluor®488 Goat Anti-mouse IgG at 1/200 dilution.
Flow Cytometry - HARS antibody [HARSA6] (ab50835)
![Flow Cytometry - HARS antibody [HARSA6] (ab50835)](/ps/datasheet/Images/50/ab50835/ab50835_4.jpg)
ab50835 at a 1/10 dilution staining HARS using Alexa Fluor® 488 Goat Anti-mouse IgG at 1/400 dilution.
Western blot - HARS antibody [HARSA6] (ab50835)
![Western blot - HARS antibody [HARSA6] (ab50835)](/ps/datasheet/Images/50/ab50835/ab50835_5.jpg)
Anti-HARS antibody [HARSA6] (ab50835) at 1/100 dilution + F2408 whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size : 46 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HARS antibody [HARSA6] (ab50835)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HARS antibody [HARSA6] (ab50835)](/ps/datasheet/images/50/ab50835/HARS-Primary-antibodies-ab50835-1.jpg)
IHC image of ab50835 staining in human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50835, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab50835 has not yet been referenced specifically in any publications.
Publishing research using ab50835? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
![Western blot - HARS antibody [HARSA6] (ab50835)](/ps/datasheet/Images/50/ab50835/ab50835_1.jpg)
Anti-HARS antibody [HARSA6] (ab50835) at 1/100 dilution + Hela whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size : 46 kDa
Observed band size : 50,100 kDa (why is the actual band size different from the predicted?)
![Immunocytochemistry - HARS antibody [HARSA6] (ab50835)](/ps/datasheet/Images/50/ab50835/ab50835_2.jpg)
ab50835 at a 1/10 dilution staining HARS in Hela cells, using Alexa Fluor®488 Goat Anti-mouse IgG at 1/200 dilution.
![Flow Cytometry - HARS antibody [HARSA6] (ab50835)](/ps/datasheet/Images/50/ab50835/ab50835_4.jpg)
ab50835 at a 1/10 dilution staining HARS using Alexa Fluor® 488 Goat Anti-mouse IgG at 1/400 dilution.
![Western blot - HARS antibody [HARSA6] (ab50835)](/ps/datasheet/Images/50/ab50835/ab50835_5.jpg)
Anti-HARS antibody [HARSA6] (ab50835) at 1/100 dilution + F2408 whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size : 46 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HARS antibody [HARSA6] (ab50835)](/ps/datasheet/images/50/ab50835/HARS-Primary-antibodies-ab50835-1.jpg)
IHC image of ab50835 staining in human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50835, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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