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Western blot - HARS antibody (ab55233)
Western blot - HARS antibody (ab55233)
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Product Name 

HARS antibody

 

See all HARS antibodies (5)...

Product type 

Primary antibodies

Description 

Mouse monoclonal to HARS

Immunogen 

Recombinant fragment: MAERAALEEL VKLQGERVRG LKQQKASAEL IEEEVAKLLK LKAQLGPDES KQKFVLKTPK GTRDYSPRQM AVREKVFDVI IRCFKRHGAE VIDTPV, corresponding to amino acids 1-97 of Human HARS
BLAST 'MAERAALEEL VKLQGERVRG LKQQKASAEL IEEEVAKLLK LKAQLGPDES KQKFVLKTPK GTRDYSPRQM AVREKVFDVI IRCFKRHGAE ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Immunology >> Immune System Diseases >> Autoimmune

Relevance 

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

 

Alternative names HARS antibody (ab55233)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

3035    (Human)

Omim

    

142810    (Human)

SwissProt

    

P12081    (Human)

Unigene

    

528050    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Isotype 

IgG2a

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: None
PBS, pH 7.2

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HARS antibody is on this datasheet. But please do contact us if you would like any reassurance!


 

HARS antibody images:

Product image  Western blot - HARS antibody (ab55233)

Western blot - HARS antibody (ab55233)



Predicted band size : 51 kDa


HARS antibody (ab55233) at 1ug/lane + HeLa cell lysate at 25ug/lane.

 
 

Search PubMed (MEDLINE) for references to HARS

 

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