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Products:Immunology >> Immune System Diseases >> Autoimmune
Overview
Product name
Anti-HARS antibody
See all HARS products (5) ...
Description
Rabbit polyclonal to HARS
Tested applications
ELISA, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide derived from C-terminal of human HARS.
Positive control
HeLa cells. HepG2 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Immunology >> Immune System Diseases >> Autoimmune
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
ICC/IF: 1/500 - 1/1000.
WB: 1/500 - 1/1000.Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Target
Relevance
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
Cellular localization
Cytoplasmic
Alternative names
- EC 6.1.1.21 antibody
- FLJ20491 antibody
- HisRS antibody
see all
Database links
- Entrez Gene: 3035 Human
- Entrez Gene: 15115 Mouse
- Omim: 142810 Human
- SwissProt: P12081 Human
- SwissProt: Q61035 Mouse
- SwissProt: Q8VDX8 Mouse
- Unigene: 528050 Human
Anti-HARS antibody images:
Immunocytochemistry/ Immunofluorescence - HARS antibody (ab65014)
ab65014 at 1/500 - 1/1000 dilution staining HARS in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Western blot - HARS antibody (ab65014)
All lanes : Anti-HARS antibody (ab65014) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 56 kDa
Observed band size : 56 kDa
Additional bands at : 20 kDa,37 kDa,40 kDa. We are unsure as to the identity of these extra bands.
References for Anti-HARS antibody (ab65014)
ab65014 has not yet been referenced specifically in any publications.
Publishing research using ab65014? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"