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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab77034? Please let us know so that we can cite the reference in this datasheet
ab77034 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - HARS antibody (ab77034)
Anti-HARS antibody (ab77034) at 1/500 dilution + HeLa cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 56 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
![Western blot - HARS antibody [Not required] (ab77034)](/ps/datasheet/Images/77/ab77034/ab77034_WB_2.jpg)
Western blot - HARS antibody [Not required] (ab77034)
Anti-HARS antibody (ab77034) at 1/500 dilution + partial recombinant human HARS protein at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 56 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
![ELISA - HARS antibody [Not required] (ab77034)](/ps/datasheet/Images/77/ab77034/ab77034_sEL_1.jpg)
ELISA - HARS antibody [Not required] (ab77034)
Sandwich ELISA using ab77034 at 1µg/mL, 100µL/well and recombinant HARS protein. HRP conjugated goat anti-rabbit IgG (H+L) used as secondary antibody.
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