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Anti-HARS antibody (ab77034)

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Overview

Product name

Anti-HARS antibody
See all HARS products (5) ...

Description

Mouse monoclonal to HARS

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Hamster, Cow, Cynomolgus Monkey, Orangutan

Immunogen

Recombinant fragment: MAERAALEEL VKLQGERVRG LKQQKASAEL IEEEVAKLLK LKAQLGPDES KQKFVLKTPK GTRDYSPRQM AVREKVFDVI IRCFKRHGAE VIDTPV, corresponding to amino acids 1-96 of human HARS (NP_002100) with a 26 kDa tag

MAERAALEEL VKLQGERVRG LKQQKASAEL IEEEVAKLLK LKAQLGPDES KQKFVLKTPK GTRDYSPRQM AVREKVFDVI IRCFKRHGAE VIDTPV

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HeLa cytoplasmic lysate; partial recombinant human HARS (the immunogen).

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 1X PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Isotype

IgG2a

Light chain type

kappa

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Immunology >> Immune System Diseases >> Autoimmune

  • Western blot - HARS antibody (ab77034)Western blot - HARS antibody (ab77034) image (enlarge)

  • Western blot - HARS antibody [Not required] (ab77034)Western blot - HARS antibody [Not required] (ab77034) image (enlarge)

  • ELISA - HARS antibody [Not required] (ab77034)ELISA - HARS antibody [Not required] (ab77034) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab77034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Cellular localization

Cytoplasmic

Alternative names

  • EC 6.1.1.21 antibody
  • FLJ20491 antibody
  • HisRS antibody
  • histidine translase antibody
  • Histidine tRNA ligase antibody
  • Histidyl tRNA synthetase antibody
  • HRS antibody
  • Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds antibody
see all

Database links

see all

Anti-HARS antibody images:

  Western blot - HARS antibody (ab77034)

Western blot - HARS antibody (ab77034)

Anti-HARS antibody (ab77034) at 1/500 dilution + HeLa cell lysate at 25 µg

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size : 56 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)

  Western blot - HARS antibody [Not required] (ab77034)

Western blot - HARS antibody [Not required] (ab77034)

Anti-HARS antibody (ab77034) at 1/500 dilution + partial recombinant human HARS protein at 0.2 µg

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Predicted band size : 56 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)

  ELISA - HARS antibody [Not required] (ab77034)

ELISA - HARS antibody [Not required] (ab77034)

Sandwich ELISA using ab77034 at 1µg/mL, 100µL/well and recombinant HARS protein. HRP conjugated goat anti-rabbit IgG (H+L) used as secondary antibody.

References for Anti-HARS antibody (ab77034)

ab77034 has not yet been referenced specifically in any publications.

Publishing research using ab77034? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"