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ab32230 |
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Anti-HAS3 antibody (ab32231) at 1 µg/ml + CHO-K1 (whole) cell lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 63 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Anti-HAS3 antibody (ab32231) at 1 µg/ml + Liver (Mouse) Tissue Lysate - normal tissue at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size : 63 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
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