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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-HAS3 antibody
Description
Rabbit polyclonal to HAS3
Tested applications
IHC-FoFr, WBmore details
Cross reactivity
Reacts with
Mouse, Chinese Hamster
Predicted to work with
Rat, Human, Pig
Immunogen
Synthetic peptide derived from within residues 500 to the C-terminus of Mouse HAS3.
(Peptide available as ab32230.)
Positive control
ab32231 gave a positive result in CHO-K1 whole cell lysate and Mouse Liver tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
WB
Application notes
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Plays a role in hyaluronan/hyaluronic acid (HA) synthesis.
Pathway
Glycan biosynthesis; hyaluronan biosynthesis.
Sequence similarities
Belongs to the nodC/HAS family.
Cellular localization
Membrane.
Target information above from: UniProt accessionO00219
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HA synthase 3 antibody
- HAS3 antibody
- HAS3_HUMAN antibody
see all
Database links
- Entrez Gene: 3038 Human
- Entrez Gene: 15118 Mouse
- Entrez Gene: 408053 Pig
- Entrez Gene: 266805 Rat
- Omim: 602428 Human
- SwissProt: O00219 Human
- SwissProt: Q8WTZ0 Human
- SwissProt: Q96RV2 Human
see all
Anti-HAS3 antibody images:
Western blot - HAS3 antibody (ab32231)
Anti-HAS3 antibody (ab32231) at 1 µg/ml + CHO-K1 (whole) cell lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 63 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Western blot - HAS3 antibody (ab32231)
Anti-HAS3 antibody (ab32231) at 1 µg/ml + Liver (Mouse) Tissue Lysate - normal tissue at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size : 63 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
References for Anti-HAS3 antibody (ab32231)
This product has been referenced in:
- Karousou Eet al. The activity of hyaluronan synthase 2 is regulated by dimerization and ubiquitination. J Biol Chem 285:23647-54 (2010). WB.Read more (PubMed: 20507985) »
See 1 publication for this product
Publishing research using ab32231? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"