Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
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ab4080 has been referenced in 4 publications.
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Predicted band size : 128 kDa
Western blot using ab4080 at 1/500. Secondary ab6721 at 1/5000. Exposure time 30s.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: MCF7
Lane 5: 3T3
Lane 6: Hela nuclear + ab11707 HAUSP peptide (aa30-41)
Lane 7: Hela + ab11707 HAUSP peptide (aa30-41)
Lane 8: A431 + ab11707 HAUSP peptide (aa30-41)
Lane 9: MCF7 + ab11707 HAUSP peptide (aa30-41)
Lane 10: 3T3 + ab11707 HAUSP peptide (aa30-41)
ICC/IF image of ab4080 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4080, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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