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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
Overview
Product name
Anti-HAUSP / USP7 antibody
See all HAUSP / USP7 products (9) ...
Description
Rabbit polyclonal to HAUSP / USP7
Specificity
By Western blot, this antibody detects a single clean band of about 130kD representing HAUSP in various cell lysates (see picture). The band is strongly depleted using peptide blocking with the immunising peptide.
Tested applications
ELISA, WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: TDDPPRITQNPVC, corresponding to amino acids 30-41 of Human HAUSP.
TDDPPRITQN PVC
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> Deubiquitination
Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Deubiquitination
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/15000 - 1/60000.
WB: 1/500 - 1/2000.Detects a band of approximately 130 kDa (predicted molecular weight: 128 kDa).
ICC/IF: Use a concentration of 1 µg/ml
IHC-P: 1/500(PubMed: 16450335)
Target
Function
Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, PTEN and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection.
Tissue specificity
Widely expressed. Overexpressed in prostate cancer.
Sequence similarities
Belongs to the peptidase C19 family.
Contains 1 MATH domain.
Domain
The C-terminus plays a role in its oligomerization.
Post-translational
modifications
Isoform 1: Phosphorylated. Isoform 1 is phosphorylated at positions Ser-18 and Ser-963. Isoform 2: Not phosphorylated.
Isoform 1: Polyneddylated. Isoform 2: Not Polyneddylated.
Isoform 1 and isoform 2: Not sumoylated.
Isoform 1 and isoform 2: Polyubiquitinated by herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110; leading to its subsequent proteasomal degradation. Isoform 1: Ubiquitinated at Lys-869.
Cellular localization
Nucleus. Cytoplasm. Nucleus > PML body. Present in a minority of ND10 nuclear bodies. Association with ICP0/VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10. Colocalizes with ATXN1 in the nucleus. Colocalized with DAXX in speckled structures. Colocalized with PML and PTEN in promyelocytic leukemia protein (PML) nuclear bodies.
Target information above from: UniProt accessionQ93009
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Deubiquitinating enzyme 7 antibody
- herpes virus associated antibody
- Herpes virus associated ubiquitin specific protease antibody
see all
Database links
- Entrez Gene: 7874 Human
- Omim: 602519 Human
- SwissProt: Q93009 Human
- Unigene: 386939 Human
Anti-HAUSP / USP7 antibody images:
Western blot - HAUSP / USP7 antibody (ab4080)
Predicted band size : 128 kDa
Western blot using ab4080 at 1/500. Secondary ab6721 at 1/5000. Exposure time 30s.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: MCF7
Lane 5: 3T3
Lane 6: Hela nuclear + ab11707 HAUSP peptide (aa30-41)
Lane 7: Hela + ab11707 HAUSP peptide (aa30-41)
Lane 8: A431 + ab11707 HAUSP peptide (aa30-41)
Lane 9: MCF7 + ab11707 HAUSP peptide (aa30-41)
Lane 10: 3T3 + ab11707 HAUSP peptide (aa30-41)
Immunocytochemistry/ Immunofluorescence - HAUSP / USP7 antibody (ab4080)
ICC/IF image of ab4080 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4080, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-HAUSP / USP7 antibody (ab4080)
This product has been referenced in:
- Huang Zet al. Deubiquitylase HAUSP stabilizes REST and promotes maintenance of neural progenitor cells. Nat Cell Biol 13:142-52 (2011). WB; Human.Read more (PubMed: 21258371) »
- Maertens GNet al. Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation of the INK4a tumour suppressor. EMBO J 29:2553-65 (2010). WB; Human.Read more (PubMed: 20601937) »
See all 4 publications for this product
Publishing research using ab4080? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"